Detection and Genotyping of Methicillin Resistance Staphylococcus aureus Infection and Colonization of Surgery Patients

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major health hazards responsible for a large number of nosocomial (hospital acquired) infections worldwide and became of greater public health concern since the emergence of community acquired MRSA. Out of 338 Staphylococcus aureus isolates collected from 374 patients, 108 (32.2 %) showed positive growth on Oxacillin Resistance Screening Agar Base (ORSAB) selective media for MRSA while only 105 (31.1%) isolates showed resistance to 30 µg cefoxitin susceptibility test and classified as MRSA. Among the MRSA isolates (105), 77 (73.3%) were identified as community acquired (CA) MRSA and 28 (26.7%) isolates were hospital acquired (HA) MRSA. The frequency of resistant and susceptibility towards 19 antibiotics revealed that all MRSA (100%) isolates were sensitive to vancomycin (VA 30 µg) and linezolid (LZD 30 µg). All of CA-MRSA and HA-MRSA isolates were screened for mecA, femA, femB, lukS-PV and lukF-PV (PVL) genes an