"Biophysical Estimation of Topically Applied Antioxidant Liposomes as Suppressor of Selenite –Induced Cataract in Experimental Animals"

Abstract

"Biophysical Estimation of Topically Applied Antioxidant Liposomes as Suppressor of Selenite –Induced Cataract in Experimental Animals" Maha Reda Ahmed Lutfy Elsyed, M. Sc. in Biophysics, 2010 In this study we divided the work into two parts; The first part: physical study on CoQ10 which used as drug into DPPC liposomes The molecular interaction between DPPC liposomes and CoenzymeQ10 (CoQ10 as drug) has been examined using differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR). It has been observed using DSC that the mixtures of DPPC and CoQ10 show a single peak, which indicates that they are miscible. The results obtained indicate that the greater effect of CoQ10 upon the incorporation into negatively charged liposomes in comparison with the other liposome formulations. The incorporated CoQ10 is probably associated with the lipid bilayers, interacted to a large extent with them, and perturbed them which results in the strong broadening and shift to lower temperature 94 C of the major characteristic endothermic peak of pure DPPC that exists at 105 C. It has been shown by means of FTIR that the incorporation of CoQ10 into DPPC induced a conformational change in the polar region of DPPC as deduced from the changes observed in the frequencies of the phosphate (PO2 -) group in the polar head of DPPC and C=O group near the head group of phospholipids in interfacial region.