Hormonal and Biochemical Effects of Omega-3 on Albino Rats Following Treatment with Some Water Disinfection By Products (DBPs)

Abstract

Infertility is a worldwide problem. Since the mid-20th century, numerous studies have reported an increasing incidence of human reproductive diseases and a consequent decline in fertility worldwide globally, it is estimated that 60 to 80 million couples are diagnosed with infertility every year. Although relatively little is known about factors affecting fertility, a growing body of scientific research indicates that some substances called "endocrine disruptors chemical (EDCs)" in the environment may interfere with the normal functions of the endocrine system and disturb male and female fertility. The main route of exposure to these chemicals is drinking water. So the present work is designed to investigate harms of tap water EDCs on (male and female fertility, bone health pituitary thyroid axis, pituitary adrenal axis & lipid profile) and role of salmon diet to relive these harms. SUMMARY 272  Summary Male and female Wister albino rats (21-28 days old) with average body weight (55-60) gm obtained from the private market Abou-Rawash, Giza, Egypt, were used in the present study. Each sex was randomly divided into 5 groups. 1st group weaning control group (n=20): after weaning animals were kept on AIN-93G and purified water ad-libitum for two months. N.B. tap water was purified by "five-stage water filtration system" to decrease or remove DBPs and estrogen from water. 2nd group adult control group (n=20): animals of 1st group were kept on purified water and AIN-93G diet ad-libitum for an extra three months which make a total of five months. 3 rd group weaning tap water treated group (n= 40): after weaning animals received tap water and AIN-93G diet ad-libitum for two months. 4th group adult tap water treated group (n=20): half the animals of 3 rd group received tap water and AIN-93G diet ad-libitum for three months which make a total of five months. 5 th group salmon diet treated group (n=20): the other half rats of 3th group received tap water and cooked salmon diet 0.34-0.69 gm / rat which is equivalent to 200 gm / 70 kg human body weight then were all given AIN-93G diet ad-libitum for three months. Raw salmon fish which import from Norway was firstly eviscerated to separate the head, fins, tail, viscera, skin and 273  Summary backbone and then the body cavity of the fish was washed with tap water to remove any traces of blood, then salmon fish was roasted in a conventional gas oven at 180 oC for 30 min.