DETECTION OF FETAL DNA BY POLYMERASE CHAIN REACTION IN MATERNAL BLOOD
Mariam Onsy Farag;
Abstract
Research in prenatal genetic diagnosis has been characterized by a focus on the development of safe and accurate alternatives to current invasive procedures. On the one hand the need was arising to ensure the birth of normal progeny in all cases, while on the other, it was not possible to eliminate the risks connected with the invasiveness of the techniques.
In human pregnancy, multiple:lines of evidence have indicated that there is bidirectional trafficking of cell free DNA and nucleated cells between the mother and fetus. The notion that the fetus and mother are in separate compartments with a relatively impermeable wall, the placenta, between them has been eradicated. Diagnostically, cell free fetal DNA in maternal plasma and fetal cells in maternal blood would offer a noninvasive source of fetal genetic material for prenatal diagnosis. Through the development of methods for fetal DNA detection and fetal cell isolation, many fetal genetic characteristics and chromosomal abnormalities could be detected from maternal blood.
In the present study, the quest for fetal genetic material in the maternal blood was pursued. Twenty one maternal blood samples at gestational ages ranging from 10 to 26 weeks of gestation were analysed. Cell free fetal DNA was detected in maternal pl,asma by Sex Determining Region Y sequence specific nested polymerase chain reaction. The Y DNA sequence from a male fetus has been used as a model system to detect fetal DNA in maternal plasma because of the obvious lack of this sequence in the mother. Accurate fetal gender diagnosis was achieved in
81.0% of pregnant women with a false negative rate of 19.0%.
In human pregnancy, multiple:lines of evidence have indicated that there is bidirectional trafficking of cell free DNA and nucleated cells between the mother and fetus. The notion that the fetus and mother are in separate compartments with a relatively impermeable wall, the placenta, between them has been eradicated. Diagnostically, cell free fetal DNA in maternal plasma and fetal cells in maternal blood would offer a noninvasive source of fetal genetic material for prenatal diagnosis. Through the development of methods for fetal DNA detection and fetal cell isolation, many fetal genetic characteristics and chromosomal abnormalities could be detected from maternal blood.
In the present study, the quest for fetal genetic material in the maternal blood was pursued. Twenty one maternal blood samples at gestational ages ranging from 10 to 26 weeks of gestation were analysed. Cell free fetal DNA was detected in maternal pl,asma by Sex Determining Region Y sequence specific nested polymerase chain reaction. The Y DNA sequence from a male fetus has been used as a model system to detect fetal DNA in maternal plasma because of the obvious lack of this sequence in the mother. Accurate fetal gender diagnosis was achieved in
81.0% of pregnant women with a false negative rate of 19.0%.
Other data
| Title | DETECTION OF FETAL DNA BY POLYMERASE CHAIN REACTION IN MATERNAL BLOOD | Other Titles | استكشاف " دى . ان . ايه " الجنين فى دم الام عن طريق تفاعل البلمرة المتسلسل | Authors | Mariam Onsy Farag | Issue Date | 2002 |
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