COMPARATIVE STUDY OF DIFFERENT METHODS OF PREPARATIONS OF PLATELET RICH PLASMA

Abstract

SUMMARY AND CONCLUSION T his study was conducted on 40 healthy adult subjects; divided into four equal groups to evaluate four different methods of preparing PRP; three double spin method, and a single spin method. From each subject, 200cc venous blood were withdrawn into plastic bags containing ACD as anticoagulant., and then undergone Cbc and platelet count, remaining blood was divided into four 40cc fractions subjected to each protocol of prp preparation. Plasma yielded was subjected to light microscopic examination, assessment of platelet function (adp aggregation test), scanning electron microscope to show ultrastructural integrity of platelets. There were in significant difference between the four studied groups as regarding platelets count. Microscopic examination SEM of group 2 and 4 (Nahita and Kececi methods) samples showed the platelets were rounded with intact continuous membrane, meanwhile SEM of the other groups1 and 3 (ACE and Tozum methods) stated that platelets were deformed and compacted, showing aggregation. Platelet function testing was preserved in the second and fourth (Nahita and Kececi methods) while in the first and third method (ACE and Tozum method) were poor and undetectable. Single centrifugation (Nahita) and double centrifugation (Kececi), both methods conserved the function and integrity of the platelets with no significance difference in platelet count between them.