PHYSIOLOGY AND PRODUCTION OF INULINASE AND GLUCOAMYLASE ENZYMES OF RHIZOPUS OLIGOSPORUS

Abstract

The present study was devoted to investigate some I physiological aspects of R.oligosporus in relation to the production of inulinase and giudoamylase having applied and potential industrial importance I

• Five different Rhizopus species <tultures were screened on I potato starch medium for glucoamylase act1v1ty. R..oligosporus DSM 1964 prodJced the highest levels of extracellular glucoamylase activi y. Thus, this culture (R. oligosporus DSM 1964) was used in the subsequent studies. I • Different byproducts were used as substitutes for starch in the growth media for gluc amylase production by R.oligosporus DSM 1964.

• R.oligusporus DSM 164 producdd maximum glucoamylase

activity when grown on either com flour or on corn bran - I lower enzyme production was 'obtained when grown on

wheat bran or sorghum bran. I

• The optimization of the culture onditions was carried out.

Increase in enzyme activity production was observed only in I media supplemented with 1% xylose or maltose or dextrin instead of starch. Increase in codcentration over 1% resulted in a notable decrease of glucoamylase production. Generally, addition of sugars repressed enzyme production.