EPIDEMIOLOGICAL STUDIES ON SALMONELLAE IN POULTRY

Abstract

A survey on Salmonella infection was carried out in broiler, balady chickens, layer chickens, non-fertile eggs, dead-in-shell chick embryos, ducks, duck eggs, rats and role of it in transmission of Salmonella in farms, feed and feed ingredients. The pathogenicity of different Salmonella isolates of different organs, organ distribution and shedding, effect: of chemotherapeutic agents and characterization of different Salmonella isolates was studied. As well as diagnosis of Salmonella infection in chickens using bacleriological methods and PCR was evaluated. The results are summarized in the following: • Out of 150 broiler flocks, 16 balady flocks, 19 layer flocks, 40 non-fertile eggs from five farms, 43 dead-in-shell chick embryos, 10 duck farms, 20 duck eggs, 16 rats and 55 feed and feed ingredients were examined and yielded 14 Salmonella isolates. • The 14 Salmonella isolates were serotyped and revealed the following: 3 isolates S. enteritidis (21.42%), S. typhimuriwn 6 isolates (42.85%), S. kentucky 2 isolates (14.28%), S. montevideo 2 isolates ( 14.28%) and 1 isolate untyped (7. 1%) .

• Using different selective enriched media for isolation of Salmonella; selenite F broth used in examination of 1 00 samples, tetrathionate brilliant green broth for 150 samples and delayed secondary enrichment used for J 19 samples, the positive samples for Salmonella were as follows 5, 2 and 5.04%, respectively.