STUDIES ON THE PRODUCTION, PREPARATION AND IMMOBILIZATION OF MICROBIAL CYCLODEXTRIN GLUCANOTRANSFERASE

Abstract

The present work deals with physiological and biochemical studies on microbial CGTase by the immobilized cells of different bacterial cultures on different carries. The productivity of CGTase was evaluated usmg the immobilized cells of Bacillus amyloliquifaciens in batch, repeated batch, and continuos cultures in t1uidized bed and packed bed bioreactor. The studies also include the immobjlization of partially purified enzyme on different carries using different immobilization techniques. The properties of the free and immobilized enzymes were compared.

The first part of this work was undertaken to study the production of CGTase by the immobilized cells of five bacterial cultures (Bacillus amyloliquf/ixiens (312), Bacillus macerans (314), Bacillus macerans (394), Bacillus macerans (3168), and Bacillus megaterium NRC4) on different immobilized matrices (i.e., agar, ca­ alginate, and polyacrylamide). In another set of experiments, the same amount of the free cells from each culture was inoculated along with the same amount of the immobilized cells and the enzyme productivity were compared.

Of all preparations, the cells of Bacillus amyloliquffaciens 312 entrapped inCa-alginate showed the highest CGTase activity (70.8 Ul ml) and the highest specific productivity (295 U I g dry weight cells I h)( Table 2). Microscopic .examination of the produced cyclodextrin intidcated that are• Cyclodextrin produces.