MOLECULAR FINGERPRINTING OF SALMONELLA TYPHI USING THE 1S200 AS EPIDEMIOLOGICAL MARKER.
Mona George Farag;
Abstract
Salmonella are gram-negative rods that cause enterocolitis, enteric fevers such as typhoid fever and septicemia.
-Over one-third of human outbreaks of food borne diseases are due to
Salmonellas.
-Typhoid fever represents a major public health concerns. It has a large and economic impact.
-The epidemiology of Salmonella infection is related to the ingestion of food and water contaminated by human and animal wastes.
-The most reliable method of diagnosis of typhoid fever is the isolation of the incriminated pathogens through culturing of blood, urine and stool. Other methods of diagnosis include detection of antibodies in sera and antigens in body fluids.
-Plasmid fingerprinting has been used for the identification of bacterial strains involved in epidemics, through evaluation the degree ofiS200 polymorphism in S. typhi isolated from typhoid patients, with respect to copy numbers and locations of insertion element in the bacterial genome.
-The integration sites of the IS200 mobile element is characterized by an inverse PCR-based assay. Inverse PCR is a useful technique for amplifying unknown DNA sequences.
So, PCR is important for molecular typing of Salmonella typhi, and is potentially useful in studying the epidemiology of typhoid fever. The IS200 typing is essential for characterizing the potential causes and the
-Over one-third of human outbreaks of food borne diseases are due to
Salmonellas.
-Typhoid fever represents a major public health concerns. It has a large and economic impact.
-The epidemiology of Salmonella infection is related to the ingestion of food and water contaminated by human and animal wastes.
-The most reliable method of diagnosis of typhoid fever is the isolation of the incriminated pathogens through culturing of blood, urine and stool. Other methods of diagnosis include detection of antibodies in sera and antigens in body fluids.
-Plasmid fingerprinting has been used for the identification of bacterial strains involved in epidemics, through evaluation the degree ofiS200 polymorphism in S. typhi isolated from typhoid patients, with respect to copy numbers and locations of insertion element in the bacterial genome.
-The integration sites of the IS200 mobile element is characterized by an inverse PCR-based assay. Inverse PCR is a useful technique for amplifying unknown DNA sequences.
So, PCR is important for molecular typing of Salmonella typhi, and is potentially useful in studying the epidemiology of typhoid fever. The IS200 typing is essential for characterizing the potential causes and the
Other data
| Title | MOLECULAR FINGERPRINTING OF SALMONELLA TYPHI USING THE 1S200 AS EPIDEMIOLOGICAL MARKER. | Other Titles | دراسة الطبعة الجزيئية لبكتريا السلمونيلا تيفود باستخدام العنصر المتوالى 200 كدليل للوباء | Authors | Mona George Farag | Issue Date | 1999 |
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