Potential Impact of a Synthesized Nanocomposite Material in Treatment of Different Forms of Liver Injury in Rats
Basma El-Soudy Abdel- Maksoud El-Agamy;
Abstract
Nanotechnology research is booming worldwide, and the general belief is the medical and biological applications will form the greatest sector of expansion over the next decade. In this view, the current study was designed to evaluate the therapeutic effect of a synthesized tricalcium phosphate nanocomposite material (nano-TCP) on different forms of liver injury such as hepatocellular carcinoma, hepatotoxicity and hepatitis.
A population of rats were divided into groups according to the treatments received. The first group injected with diethylnotrosamine intraperiteonally trice weekly at a dose level of 50 mg/kg b.w. for two weeks to initiate hepatocellular carcinoma. Then, the animals stayed three weeks without treatment followed by promotion step using 0.05 g% of phenoparbital through drinking water for another six weeks. The second group received intraperitoneal injection of 7.7 mg/kg b.w. lead nitrate trice weekly for two weeks to induce hepatotoxicity. The third group exposed to different doses of whole body γ-irradiation (6, 8 and 10 Gy) to cause efficiently hepatitis with different degrees. At the end of each treatment, 100 mg/kg b.w. of nano-TCP was injected intraperitoneally into rats three times for one week. Then, liver samples were collected for DNA fragmentation, biochemical and histopathological analyses. The biochemical parameters included the activities of hepatic alanine aminotransferase (ALT), gamma glutamyl transferase (γ-GT) as well as antioxidant status such as levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA). Also, the contents of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), heat shock protein-70 (HSP-70), matrix metalloproteinase-9 (MMP-9) and caspase-3 were accessed.
The results revealed that induction of liver injury using different insults including: DEN, lead nitrate and γ-irradiation caused high degree of DNA fragmentation accompanied by significant decrease in the levels of hepatic ALT, SOD and GPx. On the other hand, there were a significant increase in hepatic γ-GT, MDA, IL-2, IFN-γ, TNF-α, MMP-9, HSP-70, and caspase-3. The treatment with nano-tricalcium phosphate repairs efficiently the fragmented DNA and ameliorate most of the investigated parameters. The histopathological observations of liver tissues were in agreement with restored biochemical results.
In conclusion, nano-tricalcium phosphate was succeeded to handle various liver injuries beginning of inflammation through fibrosis, and even cancer. Such results present a new hope for patients with liver diseases to get treatment safe, fast and effective.
A population of rats were divided into groups according to the treatments received. The first group injected with diethylnotrosamine intraperiteonally trice weekly at a dose level of 50 mg/kg b.w. for two weeks to initiate hepatocellular carcinoma. Then, the animals stayed three weeks without treatment followed by promotion step using 0.05 g% of phenoparbital through drinking water for another six weeks. The second group received intraperitoneal injection of 7.7 mg/kg b.w. lead nitrate trice weekly for two weeks to induce hepatotoxicity. The third group exposed to different doses of whole body γ-irradiation (6, 8 and 10 Gy) to cause efficiently hepatitis with different degrees. At the end of each treatment, 100 mg/kg b.w. of nano-TCP was injected intraperitoneally into rats three times for one week. Then, liver samples were collected for DNA fragmentation, biochemical and histopathological analyses. The biochemical parameters included the activities of hepatic alanine aminotransferase (ALT), gamma glutamyl transferase (γ-GT) as well as antioxidant status such as levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA). Also, the contents of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), heat shock protein-70 (HSP-70), matrix metalloproteinase-9 (MMP-9) and caspase-3 were accessed.
The results revealed that induction of liver injury using different insults including: DEN, lead nitrate and γ-irradiation caused high degree of DNA fragmentation accompanied by significant decrease in the levels of hepatic ALT, SOD and GPx. On the other hand, there were a significant increase in hepatic γ-GT, MDA, IL-2, IFN-γ, TNF-α, MMP-9, HSP-70, and caspase-3. The treatment with nano-tricalcium phosphate repairs efficiently the fragmented DNA and ameliorate most of the investigated parameters. The histopathological observations of liver tissues were in agreement with restored biochemical results.
In conclusion, nano-tricalcium phosphate was succeeded to handle various liver injuries beginning of inflammation through fibrosis, and even cancer. Such results present a new hope for patients with liver diseases to get treatment safe, fast and effective.
Other data
| Title | Potential Impact of a Synthesized Nanocomposite Material in Treatment of Different Forms of Liver Injury in Rats | Other Titles | التأثير المحتمل لمركب مخلق بحجم النانو في علاج أنواع مختلفة من إصابات الكبد في الجرذان | Authors | Basma El-Soudy Abdel- Maksoud El-Agamy | Issue Date | 2014 |
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