A Comparative Chemical Study Of The Bioactive Secondary Metabolites Isolated from Red Sea Organisms And The Associated Fungi
Ahmed Mohamed Essam El-Din;
Abstract
Throughout the last five decades marine natural products had been progressively developed into unique branch of natural products chemistry, with thousands of unique compounds being isolated and identified from marine organisms. During the last twenty years the research field had been directed to the investigation of the endosymbiotic fungi isolated from the inner tissues of marine organisms.
In this study chemical profiling of two endosymbiotic fungi had been performed with evaluation of biological activities of the isolated compounds. The two fungi were identified by DNA sequencing technique as Arthrinium sp. isolated from the marine sponge Sarcotrgus muscarum and Eurotiomycetes sp. isolated from the mangrove marine plant Avicennia marina.
This thesis is divided into three chapters as follows:
Chapter I: Chemical investigation of the endosymbiotic fungus Arhtrinium sp.
Chapter II: Chemical investigation of the endosymbiotic fungus Eurotiomycetes sp.
Chapter III: Biological evaluation of the isolated compounds.
Chapter I
Chemical investigation of the endosymbiotic fungus Arhtrinium sp.
The fungal material was isolated from the inner tissues of the marine sponge Sarcotragus muscarum, the pure fungal strains Arhtrinium sp.was subjected to large scale fermentation procedures on solid rice media. The culture was then extracted with Ethyl acetate, the Ethyl acetate extract was then fractionated with 90% methanol. The 90% methanol fraction was subjected to VLC column packed with silica gel and eluted with n-hexane: ethyl acetate followed by Methylene chloride :methanol to yield 16 fractions. Fractions 1,2, 3, 4, 5, 6, 8 and 11-13 were further purified through different chromatographic steps to yield 22 secondary metabolite, of which compounds 1-3 were reported for the first time. The isolated compounds were classified into :
Meroterpenoids: spiroarthrinols A-C, compounds 1-3
Polyketdies : griseofulvin and dechlorogriseofulvin, 4-5, chromones 6, anthraquinons 7-9, xanthones 10-11
Shikimate derivatives: phenyl alkyl derivatives, 12-14 and 21-22, benzoic acid derivatives 16, cinnamic acid derivatives 17
In this study chemical profiling of two endosymbiotic fungi had been performed with evaluation of biological activities of the isolated compounds. The two fungi were identified by DNA sequencing technique as Arthrinium sp. isolated from the marine sponge Sarcotrgus muscarum and Eurotiomycetes sp. isolated from the mangrove marine plant Avicennia marina.
This thesis is divided into three chapters as follows:
Chapter I: Chemical investigation of the endosymbiotic fungus Arhtrinium sp.
Chapter II: Chemical investigation of the endosymbiotic fungus Eurotiomycetes sp.
Chapter III: Biological evaluation of the isolated compounds.
Chapter I
Chemical investigation of the endosymbiotic fungus Arhtrinium sp.
The fungal material was isolated from the inner tissues of the marine sponge Sarcotragus muscarum, the pure fungal strains Arhtrinium sp.was subjected to large scale fermentation procedures on solid rice media. The culture was then extracted with Ethyl acetate, the Ethyl acetate extract was then fractionated with 90% methanol. The 90% methanol fraction was subjected to VLC column packed with silica gel and eluted with n-hexane: ethyl acetate followed by Methylene chloride :methanol to yield 16 fractions. Fractions 1,2, 3, 4, 5, 6, 8 and 11-13 were further purified through different chromatographic steps to yield 22 secondary metabolite, of which compounds 1-3 were reported for the first time. The isolated compounds were classified into :
Meroterpenoids: spiroarthrinols A-C, compounds 1-3
Polyketdies : griseofulvin and dechlorogriseofulvin, 4-5, chromones 6, anthraquinons 7-9, xanthones 10-11
Shikimate derivatives: phenyl alkyl derivatives, 12-14 and 21-22, benzoic acid derivatives 16, cinnamic acid derivatives 17
Other data
| Title | A Comparative Chemical Study Of The Bioactive Secondary Metabolites Isolated from Red Sea Organisms And The Associated Fungi | Other Titles | دراسة كيميائية مقارنة للمركبات ذات الفاعلية الحيوية المفصولة من كائنات بحرية بالبحر الأحمر و الفطريات المصاحبة لها | Authors | Ahmed Mohamed Essam El-Din | Issue Date | 2017 |
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