Isolation and identification of some halotolerant actinomycetes having antagonistic activities against some plant pathogens (ie., Tobacco mosaic virus, Aspergillus sp., Fusarium sp.) from soil of Taif Governorate, KSA

SH Mohamed, WM Omran, SM Abdelsalam, AS Al-Shehri, AS Sadik ; Abdelsalam, Shimaa 


Abstract


Actinomycetes are considered one of the important bacteria due to their ability to produce several substances as secondary metabolites, found to be effective in the control of some plant pathogens. In this study, a number of soil samples were collected from different locations of Taif as well as Jeddah and Makah. This was followed by determination of the microbial total counts, i.e., bacteria, fungi and actinomycetes. Actinomycetes were isolated and purified and their color groups were determined. The actinomycete isolates were identified based on their cultural and morphological properties as Streptomycetes. The salt tolerance range of the purified Streptomyces isolates were determined by growing them on starch nitrate agar medium supplemented with different NaCl concentrations ranged from 3.5 to 10.5%. The highly tolerant streptomycete isolates were grown on a starch nitrate broth medium for 6 days under shaking conditions at 28-30 o C. Then, their antiviral and antifungal activities against TMV, Aspergillus sp. and Fusarium sp. were determined. The halotolerant Streptomycete isolates having antifungal and/or antiviral activities were completely identified based on their cultural, morphological and physiological properties. The DNA of the identified isolates was extracted and used for determination of DNA fingerprinting of these isolates using the RAPD-PCR molecular tool.


Other data

Keywords Actinomycete, Streptomycetes, Taif region, Antiviral and antifungal activity, TMV, Aspergillus, Fusarium, RAPD-PCR.
Issue Date 2012
Publisher Institute of Biotechnology and Genetic Engineering,University of Sindh, Jamshoro, Pakistan
Source 10
Journal PAKISTAN JOURNAL OF BIOTECHNOLOGY 
URI http://research.asu.edu.eg/handle/123456789/1316


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