Isolation and identification of some halotolerant actinomycetes having antagonistic activities against some plant pathogens (ie., Tobacco mosaic virus, Aspergillus sp., Fusarium sp.) from soil of Taif Governorate, KSASH Mohamed, WM Omran, SM Abdelsalam, AS Al-Shehri, AS Sadik ; Abdelsalam, Shimaa
AbstractActinomycetes are considered one of the important bacteria due to their ability to produce several substances as secondary metabolites, found to be effective in the control of some plant pathogens. In this study, a number of soil samples were collected from different locations of Taif as well as Jeddah and Makah. This was followed by determination of the microbial total counts, i.e., bacteria, fungi and actinomycetes. Actinomycetes were isolated and purified and their color groups were determined. The actinomycete isolates were identified based on their cultural and morphological properties as Streptomycetes. The salt tolerance range of the purified Streptomyces isolates were determined by growing them on starch nitrate agar medium supplemented with different NaCl concentrations ranged from 3.5 to 10.5%. The highly tolerant streptomycete isolates were grown on a starch nitrate broth medium for 6 days under shaking conditions at 28-30 o C. Then, their antiviral and antifungal activities against TMV, Aspergillus sp. and Fusarium sp. were determined. The halotolerant Streptomycete isolates having antifungal and/or antiviral activities were completely identified based on their cultural, morphological and physiological properties. The DNA of the identified isolates was extracted and used for determination of DNA fingerprinting of these isolates using the RAPD-PCR molecular tool.
|Keywords||Actinomycete, Streptomycetes, Taif region, Antiviral and antifungal activity, TMV, Aspergillus, Fusarium, RAPD-PCR.||Issue Date||2012||Publisher||Institute of Biotechnology and Genetic Engineering,University of Sindh, Jamshoro, Pakistan||Source||10||Journal||PAKISTAN JOURNAL OF BIOTECHNOLOGY||URI||http://research.asu.edu.eg/handle/123456789/1316|
Recommend this item
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.