Evaluation of Pro-Apoptotic activity of Chelidonium majus L. And/or gamma-radiation against Ehrlich as carcinoma transplanted in mice | Avaliação da atividade pró-apoptótica da Chelidonium majus L. E/ou radiação gama contra o tumor ascítico de ehrlich transplantado em ratos
Thabet, N.M.; Moustafa, E.M.; Elkhashab, Iman;
Abstract
This study was to evaluate, the pro-apoptotic activity of ethanolic Chelidonium majus L. (CM)
leaf extract and/or gamma-radiation in Ehrlich ascites carcinoma (EAC) bearing mice by measuring tumor volume,
apoptotic factors (caspase-3, Bcl-2 and Bax proteins, tumor necrosis factor-alpha (TNF- ), angiogenesis factors (matrix
metalloproteinase (MMP-2 and MMP-9); tissue inhibitor of metalloproteinases (TIMP-1), DNA fragmentation, besides
histopathological examination. After tumor inoculation, mice received CM (100 mg/body weight/day) on the 10th day and
were exposed to whole body -radiation (6Gy) on the 17th day. The data obtained reveales that combining CM with -
radiation exposure induce significant regression in tumor growth, up-regulate Bax, caspase-3, TIMP-1 and inhibit Bcl-2,
TNF- , MMP-(2 and 9). Cytotoxicity is substantiated by increased DNA-fragmentation (comet assay and DNA content)
and histological examination. It could be suggested that combining CM with gamma-radiation increased apoptosis of
tumor cells which would help to increase the lifespan of mice.
leaf extract and/or gamma-radiation in Ehrlich ascites carcinoma (EAC) bearing mice by measuring tumor volume,
apoptotic factors (caspase-3, Bcl-2 and Bax proteins, tumor necrosis factor-alpha (TNF- ), angiogenesis factors (matrix
metalloproteinase (MMP-2 and MMP-9); tissue inhibitor of metalloproteinases (TIMP-1), DNA fragmentation, besides
histopathological examination. After tumor inoculation, mice received CM (100 mg/body weight/day) on the 10th day and
were exposed to whole body -radiation (6Gy) on the 17th day. The data obtained reveales that combining CM with -
radiation exposure induce significant regression in tumor growth, up-regulate Bax, caspase-3, TIMP-1 and inhibit Bcl-2,
TNF- , MMP-(2 and 9). Cytotoxicity is substantiated by increased DNA-fragmentation (comet assay and DNA content)
and histological examination. It could be suggested that combining CM with gamma-radiation increased apoptosis of
tumor cells which would help to increase the lifespan of mice.
Other data
Title | Evaluation of Pro-Apoptotic activity of Chelidonium majus L. And/or gamma-radiation against Ehrlich as carcinoma transplanted in mice | Avaliação da atividade pró-apoptótica da Chelidonium majus L. E/ou radiação gama contra o tumor ascítico de ehrlich transplantado em ratos | Authors | Thabet, N.M. ; Moustafa, E.M. ; Elkhashab, Iman | Keywords | Caspase-3;Bcl-2. TIMP-1;DNA fragmentation;Tumor growth | Issue Date | Nov-2016 | Journal | Bioscience Journal | DOI | http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS |
Scopus ID | 2-s2.0-85006074196 |
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