Evaluation of Pro-Apoptotic activity of Chelidonium majus L. And/or gamma-radiation against Ehrlich as carcinoma transplanted in mice | Avaliação da atividade pró-apoptótica da Chelidonium majus L. E/ou radiação gama contra o tumor ascítico de ehrlich transplantado em ratos

Thabet, N.M. ; Moustafa, E.M. ; Elkhashab, Iman 


Abstract


This study was to evaluate, the pro-apoptotic activity of ethanolic Chelidonium majus L. (CM) leaf extract and/or gamma-radiation in Ehrlich ascites carcinoma (EAC) bearing mice by measuring tumor volume, apoptotic factors (caspase-3, Bcl-2 and Bax proteins, tumor necrosis factor-alpha (TNF- ), angiogenesis factors (matrix metalloproteinase (MMP-2 and MMP-9); tissue inhibitor of metalloproteinases (TIMP-1), DNA fragmentation, besides histopathological examination. After tumor inoculation, mice received CM (100 mg/body weight/day) on the 10th day and were exposed to whole body -radiation (6Gy) on the 17th day. The data obtained reveales that combining CM with - radiation exposure induce significant regression in tumor growth, up-regulate Bax, caspase-3, TIMP-1 and inhibit Bcl-2, TNF- , MMP-(2 and 9). Cytotoxicity is substantiated by increased DNA-fragmentation (comet assay and DNA content) and histological examination. It could be suggested that combining CM with gamma-radiation increased apoptosis of tumor cells which would help to increase the lifespan of mice.


Other data

Keywords Caspase-3;Bcl-2. TIMP-1;DNA fragmentation;Tumor growth
Issue Date Nov-2016
Journal Bioscience Journal 
URI http://research.asu.edu.eg/handle/123456789/1489
DOI http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-85006074196&partnerID=MN8TOARS


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