Modulation of selenite-induced cataract by dietary supplement of broccoli in experimental animalsKassem, S.S. ; Aziz, M.A. ; Nora Afifi ; Kholeif, T.E. ; Al-Balkini, M.S. ; Gomaa, A.M. ; El-Razek, F.H. ; Hassan, H.H.
AbstractOxidative stress remains the underlying factor in many cases of cataracts. Selenite-induced cataract was worked upon as a model system for oxidative stress in rats. Naturally occurring compounds like flavonoids are known to exhibit antioxidant activity and hence, might be of potential therapeutic value. In the present study, an attempt has been made to determine the efficacy of broccoli in modulating or preventing selenite-induced cataractogenesis. Twenty seven Wister rat pups were divided into four groups. Group one (n=6) received basal diet and served as normal control group. The rats in group two (n=8) were injected subcutaneously with sodium selenite (30μmol/kg body weight) to induce cataract and fed on basal diet. Group three (n=7) normal rat pups supplemented with 10% broccoli added to their basal diet. Group four selenite-induced cataract (n=6) was also fed on 10% broccoli in their basal diet and served as treated group. Cataract was assessed one week later and its density was graded by slit lamp biomicroscopy. The identification of individual phenolic compounds of the broccoli was performed. After the end of the experiment (two months), all rats were fasted overnight. Blood samples were collected from the eye vein and then lenses were excised. All planned samples were prepared as will be described. The levels of serum lipid profiles were determined. The activity and levels of catalase, superoxide dismutase, total antioxidant capacity, reduced glutathione, malondialdehyde and nitric oxide were assessed in the blood and lens. Fas ligand (FAS-L) as apoptotic marker was also assessed in the blood and lens. On the other hand; the levels of blood hemoglobin and fasting blood sugar were determined. Ophthalmic examination revealed that, lenses in the control group were clear; but in selenite-induced cataract group, all lenses developed complete dense opacification (grade 4), whereas mild opacification was observed in the group four (after treatment). The development of cataract was reduced into 80% in the group supplemented with 10% broccoli. The consumption of 10% broccoli in cataractous group led to a significant decrease in serum total lipids, total cholesterol, triacylglycerols and low density lipoprotein- cholesterol as well as significant decrease in malondialdehyde, nitric oxide and Fas ligand levels. On the other hand, supplementation of broccoli led to a significant increase in the activities and levels of catalase, superoxide dismutase and total antioxidant capacity and reduced glutathione, respectively as compared to selenite-induced cataract group. In conclusions, the present data showed that broccoli reduces selenite-induced cataract formation by inhibiting lipid peroxidation, oxidative stress and acts as anti-apoptotic agent that can reduce or even prevent progress of cataract formation. © IDOSI Publications, 2013.
|Keywords||Broccoli, Selenite-Induced Cataract, Phenolic Compound, Oxidative Stress, Fas-L, Delay Cataract||Issue Date||2013||Journal||World Applied Sciences Journal||URI||http://research.asu.edu.eg/handle/123456789/155810||ISSN||1818-4952||DOI||http://www.scopus.com/inward/record.url?eid=2-s2.0-84890195064&partnerID=MN8TOARS
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