Isolation and Characterization of Bacillus subtilis Phage from Soil Cultivated with Liquorices Root

Marie, Eman,

Abstract


One virulent phage specific for Bacillus Subtilis was isolated from soil cultivated with liquorices
plants in Matruh governorate, Egypt. The B. subtilis phage isolate produced 0.13 cm diameter circular plaques;
turbid and without central zone. In vitro determination of thermal inactivation point (TIP), dilution end point
(DEP) and longevity in vitro (LIV) were found to be 68°C/10 min, 10-4 and 96 hrs, respectively. B. subtilis phage
isolate has a narrow host range and infected only the B. subtilis. Transmission electron microscopy examination
showed that the purified phage is a tailed, isometric shape head and the diameter was 59 nm. In protein pattern
characterization of purified B. subtilis phage isolate showed 8 structural proteins with different molecular
weights when compared with protein marker. In RAPD-PCR, only eight primers succeeded to generate
polymorphic DNA products. Thirty five bands were unique polymorphism with all primer used and twenty three
bands were monomorphic.


Other data

Title Isolation and Characterization of Bacillus subtilis Phage from Soil Cultivated with Liquorices Root
Authors Marie, Eman 
Keywords Bacillus subtilis phages - Biological properties - Protein pattern and RAPD-PCR
Issue Date 2013
Publisher IDOSI Publications, 2013
Journal International Journal of Microbiological Research, 4 (1): 43-49 
URI http://research.asu.edu.eg/handle/123456789/1608
DOI 10.5829/idosi.ijmr.2013.4.1.7231

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