Biochemical, Histological and Histochemical Changes induced in Pregnant Albino Rats as Affected by Silymarin Treatment and / or Gamma Irradiation
Amany Abdelhamied Mahmoud Osman; Ramadan, F.l.;
Abstract
Silymarin is a mixture of flavonoids extracted from seeds of milk thistle (Silybum marianum), that has been used in treatment of liver diseases. Flavonoids are a large group of polycyclic phenols of plant origin that are displaying estrogenic effects: Silybum marianum has been traditionally used in Egypt for its antifertility effect.
The objective of this study was to evaluate the side effects of silymarin administration and / or exposure to radiation in pregnant rats. Silymarin at a dose level 75.6 mg/kg body weight was daily administered via an oral stomach tube to pregnant adult albino rats from the 1st to the 20th day of pregnancy while mothers were subjected to gamma radiation (1.5 Gy) as fractionated dose 0.75 Gy on the 6th day and 0.75 Gy on 12th day of pregnancy. Experimental investigations carried out one day prior to parturition have demonstrated that silymarin intake throughout the whole gestational period induced biochemical, histolpathological and histochemical disorders in irradiated mothers. The data obtained revealed that silymarin administration and / or radiation exposure caused significant elevation in levels of follicle stimulating hormone (FSH), the luteinizing hormone (LH) and estradiol in pregnant rats.
Moreover, the histopathological results showed different distortions. These distortions varied from hyperemic blood vessels, fibroblasts in the ovary, degenerated uterine glands, erosion in the lining epithelia of the uterus and degenerated epithelial cells, necrosis in trophospongium, necrosis in the giant cells, massive blood in the labyrinth and cytoplasmic vacuolation in the placenta. In addition, the histochemical observations revealed various diminution in each of the polysaccharides, total protein and DNA content.
Conclusively, these findings proved that radiation exposure or / silymarin intake could exert deleterious effect. Therefore, it is recommended that radiation occupational workers especially females have to be careful toward silymarin intake during pregnancy.
The objective of this study was to evaluate the side effects of silymarin administration and / or exposure to radiation in pregnant rats. Silymarin at a dose level 75.6 mg/kg body weight was daily administered via an oral stomach tube to pregnant adult albino rats from the 1st to the 20th day of pregnancy while mothers were subjected to gamma radiation (1.5 Gy) as fractionated dose 0.75 Gy on the 6th day and 0.75 Gy on 12th day of pregnancy. Experimental investigations carried out one day prior to parturition have demonstrated that silymarin intake throughout the whole gestational period induced biochemical, histolpathological and histochemical disorders in irradiated mothers. The data obtained revealed that silymarin administration and / or radiation exposure caused significant elevation in levels of follicle stimulating hormone (FSH), the luteinizing hormone (LH) and estradiol in pregnant rats.
Moreover, the histopathological results showed different distortions. These distortions varied from hyperemic blood vessels, fibroblasts in the ovary, degenerated uterine glands, erosion in the lining epithelia of the uterus and degenerated epithelial cells, necrosis in trophospongium, necrosis in the giant cells, massive blood in the labyrinth and cytoplasmic vacuolation in the placenta. In addition, the histochemical observations revealed various diminution in each of the polysaccharides, total protein and DNA content.
Conclusively, these findings proved that radiation exposure or / silymarin intake could exert deleterious effect. Therefore, it is recommended that radiation occupational workers especially females have to be careful toward silymarin intake during pregnancy.
Other data
| Title | Biochemical, Histological and Histochemical Changes induced in Pregnant Albino Rats as Affected by Silymarin Treatment and / or Gamma Irradiation | Authors | Amany Abdelhamied Mahmoud Osman ; Ramadan, F.l. | Issue Date | Jul-2007 | Publisher | Middle Eastern Regional Radiosotope Center For The Arab Countries | Journal | Isotope and radiation research | Description | the present investigation showed that the deleterious influence of either silymarin or radiation exposure is highly noticed when both treatment are performed simultaneously in synergistic form. |
ISSN | 0021-1907 |
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