Interaction of Coenzyme Q10 with Liposomes and its Impact on Suppression of Selenite – Induced Experimental Cataract
Maha Reda Elsyed; Medhat Wahba Shafaa; Amany Hasan Elshazly; Amira Zaki Dakrory;
Abstract
Purpose: To stress the influence of Coenzyme Q10 (CoQ10) on the structural properties of
liposomes as model membranes and to investigate the possible role of CoQ10 or CoQ10
doped in liposomes when topically instilled as eye drops, in preventing cataract.
Methods: The molecular interaction between liposomes and Coenzyme Q10 was examined
using differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy
(FTIR). Rat pups were randomly divided into six groups comprising 15 pups. Group (1),
control group. Group (2), untreated model of cataract, received a single subcutaneous
injection of sodium selenite. Instillation of pure CoQ10 (Group 3), CoQ10 encapsulated into
neutral (Group 4), positive (Group 5) and negative (Group 6) Dipalmitoyl
phosphatidylcholine (DPPC) liposomes on the opacification of lenses in rat pups after sodium
selenite injection was topically received.
Results: The incorporated CoQ10 is probably associated with lipid bilayers where it interacts to
a large extent and perturbs them. This results in strong broadening and shift to lower temperature
(94C) of the major characteristic endothermic peak of pure DPPC at 105°C.
FTIR showed that the incorporation of CoQ10 into DPPC induces a conformational change
in the polar region of DPPC. Ophthalmological and Biochemical studies revealed that CoQ10
alone followed by negatively charged liposomes doped with CoQ10 are more effective in
reducing the progress of cataract as well as improving the lens soluble proteins levels and
total antioxidant capacity.
Conclusion: The interactions of CoQ10 with membrane systems may contribute to a better
understanding of CoQ10 physiological properties and the development of therapeutically
advanced systems.
liposomes as model membranes and to investigate the possible role of CoQ10 or CoQ10
doped in liposomes when topically instilled as eye drops, in preventing cataract.
Methods: The molecular interaction between liposomes and Coenzyme Q10 was examined
using differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy
(FTIR). Rat pups were randomly divided into six groups comprising 15 pups. Group (1),
control group. Group (2), untreated model of cataract, received a single subcutaneous
injection of sodium selenite. Instillation of pure CoQ10 (Group 3), CoQ10 encapsulated into
neutral (Group 4), positive (Group 5) and negative (Group 6) Dipalmitoyl
phosphatidylcholine (DPPC) liposomes on the opacification of lenses in rat pups after sodium
selenite injection was topically received.
Results: The incorporated CoQ10 is probably associated with lipid bilayers where it interacts to
a large extent and perturbs them. This results in strong broadening and shift to lower temperature
(94C) of the major characteristic endothermic peak of pure DPPC at 105°C.
FTIR showed that the incorporation of CoQ10 into DPPC induces a conformational change
in the polar region of DPPC. Ophthalmological and Biochemical studies revealed that CoQ10
alone followed by negatively charged liposomes doped with CoQ10 are more effective in
reducing the progress of cataract as well as improving the lens soluble proteins levels and
total antioxidant capacity.
Conclusion: The interactions of CoQ10 with membrane systems may contribute to a better
understanding of CoQ10 physiological properties and the development of therapeutically
advanced systems.
Other data
Title | Interaction of Coenzyme Q10 with Liposomes and its Impact on Suppression of Selenite – Induced Experimental Cataract | Authors | Maha Reda Elsyed ; Medhat Wahba Shafaa ; Amany Hasan Elshazly ; Amira Zaki Dakrory | Keywords | Liposomes Coenzyme Q10 DSC FTIR Cataract Lens Soluble Proteins | Issue Date | 8-Jan-2018 | Publisher | Advanced Pharmaceutical Bulletin | Journal | Advanced Pharmaceutical Bulletin | Description | biophysics research |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.