Effect of Snake Venom Disintegrin like domain on the Homing of Mesenchymal Stem Cells

Abstract

Mesenchymal stem cells have many advantages as grafts for cell transplantation. The homing of MSCs after systemic infusion is still poorly understood. This report explored the effect of the combination of BM-MSCs with Disintegrin like fraction obtained from Cerastes cerastes crude venom on the fibrotic liver of CCl4 Rtreated mice. It was observed that Disintegrin like fraction could increase homing of BM-MSCs labeled with the PKH26 into the liver tissue of CCl4 Rtreated mice. A significant decrease in AST and ALT serum levels after administration of Disintegrin like fraction and/or BM-MSCs in CCl4 treated mice were detected. VEGF was expressed in mice injected with CCl4 alone, followed by Disintegrin like fraction or both Disintegrin like fraction and BM-MSCs. β- catenin was expressed in mice injected with CCl4 alone, followed by BM-MSCs or both BM-MSCs and Disintegrin like fraction. Caspase-3 gene was expressed in CCl4 Rtreated mice injected with BM-MSCs or both BM-MSCs and Disintegrin like fraction. TNF-α and HO-1 were expressed in all groups. Administration of Disintegrin like fraction and / or BM-MSCs improved the histo-pathological picture and showed signs of regeneration in CCl4 induced liver fibrosis. In conclusion, Disintegrin like fraction could increase homing of BM-MSCs into the liver tissue of CCl4 Rtreated mice. Further studies need to be done to explore the mechanism of homing caused by Disintegrin.