The cytogenetic potential of ivermectin on bone marrow cells of mice in vivo.
Karima Mohammad Sweify Iman Abd ElMoneim Darwish Dalia Demerdash abd El-Monem; Sweify.Karima;
Abstract
:Ivermectin (IVM) is a broad-spectrum anti spectrum agent. It shows an excellent anthelmintic effect
in veterinary and human medicine. The objective of this study was the evaluation of potential cytogenicity of
IVM on Mus musculus in vivo.This was achieved through chromosomal aberration assay (CAA) and
micronucleus test (MNT) in bone marrow cells.For CAA & MNT, animals received single and/or double doses
of 200ug/kg b.w. IVM. The sampling times were 1, 2, 3, 7, and 14 days after the last injection.The maximum
values of the chromosomal aberrations percent (excluding gap) attained after 3 days, when including gap, the
readings obtained after 1, 2, 3 and 7 days of single treatment were approached to each other, then decreased
after 14 days. The chromosomal aberrations percent obtained after repeated injection showed elevation
through the first 3 days and reduction after 7 and 14 days. After repeated dosing the chromosomal aberrations
percent (CA%) reached its highest value after 2 and 3 days with including and excluding gaps, respectively.
Single dose of IVM gave high score of MN than that obtained by double doses. In both groups MNPCEs were
decreased by increasing time. On the other hand, IVM treatment induced highly significant decrease in the ratio
of PCEs/NCEs at all the experimental periods, with the exception of data obtained after 14 days.IVM induced
high level of chromosome aberrations in somatic cells, as it is ascertained by chromosome aberration assay and
micronuclei production in bone marrow cells. This study revealed high clastogenic and genotoxic potential of
IVM on mice
in veterinary and human medicine. The objective of this study was the evaluation of potential cytogenicity of
IVM on Mus musculus in vivo.This was achieved through chromosomal aberration assay (CAA) and
micronucleus test (MNT) in bone marrow cells.For CAA & MNT, animals received single and/or double doses
of 200ug/kg b.w. IVM. The sampling times were 1, 2, 3, 7, and 14 days after the last injection.The maximum
values of the chromosomal aberrations percent (excluding gap) attained after 3 days, when including gap, the
readings obtained after 1, 2, 3 and 7 days of single treatment were approached to each other, then decreased
after 14 days. The chromosomal aberrations percent obtained after repeated injection showed elevation
through the first 3 days and reduction after 7 and 14 days. After repeated dosing the chromosomal aberrations
percent (CA%) reached its highest value after 2 and 3 days with including and excluding gaps, respectively.
Single dose of IVM gave high score of MN than that obtained by double doses. In both groups MNPCEs were
decreased by increasing time. On the other hand, IVM treatment induced highly significant decrease in the ratio
of PCEs/NCEs at all the experimental periods, with the exception of data obtained after 14 days.IVM induced
high level of chromosome aberrations in somatic cells, as it is ascertained by chromosome aberration assay and
micronuclei production in bone marrow cells. This study revealed high clastogenic and genotoxic potential of
IVM on mice
Other data
| Title | The cytogenetic potential of ivermectin on bone marrow cells of mice in vivo. | Authors | Karima Mohammad Sweify Iman Abd ElMoneim Darwish Dalia Demerdash abd El-Monem ; Sweify.Karima | Keywords | Ivermectin (IVM), chromosomal aberration assay (CAA), micronucleus test (MNT), (polychromatic erythrocytes), NCEs (normochromatic erythrocytes) | Issue Date | 2015 | Publisher | Journal of Environmental Science , Toxicology and good Technology | Journal | Journal of Environmental Science , Toxicology and good Technology |
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