Evaluation differences of some ruminal bacteria by in vitro dry matter, cellulose and hemicellulose disappearance rate and extent of bagasse

Abstract

The objectives of this paper are to evaluate the differences among five cellulolytic bacteria isolated from the rumen regarding in vitro dry matter, cellulose and hemicellulose disappearance. Cellulolytic bacteria such as Cellulomonas cellulasea, Acetobacter xylinum, Thermonospora fusca, Ruminococcus albus and Bacillus sp. had been isolated from cow, sheep, buffalo and camel. Sugarcane bagasse was incubated with each strain for 48 hrs incubation (extent) and the regression coefficient of in vitro was rate of disappearance per hour. The increase recorded of in vitro dry matter disappearance values. Five strains isolated from sheep were more effective than that isolated from buffalo and cow. The results revealed that Cellulomonas and Ruminococcus isolated from camel showed the highest (p<0.05) value of in vitro cellulose disappearance of bagasse (65% and 62%). While Bacillus isolated from sheep showed the highest (p<0.05) value of in vitro cellulose and hemicellulose digestion (53% and 47%, respectively). Thermonospora and Acetobacter isolated from cow showed higher of in vitro cellulose digestion (56% and 46%) more than those isolated from camel, sheep and buffalo. The results recorded that Thermonospora isolated from cow had the highest value of in vitro hemicellulose disappearance (52%), while Cellulomonas isolated from buffalo showed the highest value (64%). Acetobacter and Ruminococcus isolated from camel showed the highest value of hemicellulose (44% and 62%). It was concluded that the five strains isolated from four ruminant animals significant differences on in vitro dry matter, cellulose and hemicellulose disappearance of sugarcane bagasse with the same strain.