Phytochemicals in Pomegranate Seeds and Their Effect as Hypolipidemic Agent in Hypercholesterolemic Rats
I.S. Ashoush; M.A. Elbandy;
Abstract
In the present study, pomegranate seed oils (PSO) were extracted and analyzed for their fatty acid
profiles and total tocopherols. The defatted pomegranate seed residue (PSR) was evaluated for its total phenolic
and scavenging capacities against DPPH. Dietary effect of pomegranate seed oil, defatted seed residue and their
mixture (PSOR) on hypercholesterolemic rats was investigated. The results revealed that, pomegranate seed
oil was rich in polyunsaturated fatty acid (88.4%) and total tocopherols (905.5 μg/100g). Meanwhile, the
pomegranate seed residue contained amount of total phenolics (14.8 mg/g) and exhibited high scavenging
activity (98.2%). After 28 days of feeding period, atherogenic rats group (AC) revealed an increasing in level
of total cholesterol, triglyceride, low density lipoprotein cholesterol (LDL-C), risk ratio, atherogenic index and
lipid peroxidation compared with normal control rats group (NC). Supplemented diet with 5% pomegranate seed
oil, 10% pomegranate seed residue and 15% of their mixture at the ratio of 2:1 respectively improved lipid profile
of plasma as compared to AC group. Furthermore, the supplementation diets induced a significant increase in
levels of reduced glutathione and a reduction in malondialdehyde level (MDA) when compared to AC group.
In addition, the histopathological examination of the heart and aorta in rats group fed on hypercholesterolemic
diet showed severe histological changes as compared to supplemented PSO, PSR and PSOR groups, these
results reflect the protective effect of pomegranate seed fractions (oil and residue) against atherosclerosis and
it have more potential as a health supplement rich in natural phytochemicals.
profiles and total tocopherols. The defatted pomegranate seed residue (PSR) was evaluated for its total phenolic
and scavenging capacities against DPPH. Dietary effect of pomegranate seed oil, defatted seed residue and their
mixture (PSOR) on hypercholesterolemic rats was investigated. The results revealed that, pomegranate seed
oil was rich in polyunsaturated fatty acid (88.4%) and total tocopherols (905.5 μg/100g). Meanwhile, the
pomegranate seed residue contained amount of total phenolics (14.8 mg/g) and exhibited high scavenging
activity (98.2%). After 28 days of feeding period, atherogenic rats group (AC) revealed an increasing in level
of total cholesterol, triglyceride, low density lipoprotein cholesterol (LDL-C), risk ratio, atherogenic index and
lipid peroxidation compared with normal control rats group (NC). Supplemented diet with 5% pomegranate seed
oil, 10% pomegranate seed residue and 15% of their mixture at the ratio of 2:1 respectively improved lipid profile
of plasma as compared to AC group. Furthermore, the supplementation diets induced a significant increase in
levels of reduced glutathione and a reduction in malondialdehyde level (MDA) when compared to AC group.
In addition, the histopathological examination of the heart and aorta in rats group fed on hypercholesterolemic
diet showed severe histological changes as compared to supplemented PSO, PSR and PSOR groups, these
results reflect the protective effect of pomegranate seed fractions (oil and residue) against atherosclerosis and
it have more potential as a health supplement rich in natural phytochemicals.
Other data
Title | Phytochemicals in Pomegranate Seeds and Their Effect as Hypolipidemic Agent in Hypercholesterolemic Rats | Authors | I.S. Ashoush ; M.A. Elbandy | Keywords | Pomegranate seed oil, Defatted residue, DPPH, Hypolipidemic, Lipid profile, Oxidative stress, Atherogenic rats | Issue Date | 2012 | Publisher | IDOSI Publications | Journal | World Journal of Dairy & Food Sciences 7 (1): 85-92 | ISSN | 1817-308X | DOI | 10.5829/idosi.wjdfs.2012.7.1.1107 |
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