Impact of 24-epibrassinolide on callogenesis and regeneration via somatic embryogenesis in Phaseolus vulgaris l. cv. Brunca

Abstract

This study established a promising protocol for callus induction and regeneration of Phaseolus vulgaris L. cv. Brunca. Whereas callus was induced on leaf, stem and root explants cultured on MS medium fortified with 1.5 mg L-12, 4-D in combination with 0.1 mg L-1of 24-Epi and leaf explants showed the best result. Direct and indirect somatic embryogenesis was achieved from stem and leaf explants while root failed in recovery with all tested media. The best performance of direct and indirect somatic embryogenesis were observed on MS medium supplemented with 2.0 mg L-1TDZ + 0.1 mg L-1of 24-Epi, while highest percentage of embryogenic frequency 88.9 and 72.23 were recorded for stem explants. However, the greatest embryogenic calli fresh weights 14.68 and 9.65 (mg/jar) were derived from leaf explants. Moreover, growth of somatic embryos was investigated during 7weeks. The highest regeneration percentage was achieved for stem explants compared other explants and recorded 88.88 and 66.66 for directly or indirectly regeneration, respectively, cultured on MS medium augmented with 2 mg L-1TDZ + 0.1 mg L-1of 24-Epi. Further, regeneration was found higher via direct embryogenesis as compared to indirect embryos formation and leaf explants were more sensitive compared to stem under the study conditions. Hence 24-Epi (0.1 mg L-1) additively improved and hastened of 2, 4-D and TDZ role in Brunca plantlets regeneration. © IDOSI Publications, 2013.