Molecular Markers Associated with Some Resistance Potato Cultivars to Bacterial Wilt Disease

Abstract

Bacterial wilt (B.W) disease caused by Ralstonia solanacearum in potato. Among six of commonlycultivated potato cultivars (Solanum tuberosum), Nicola and Lady rosetta cultivars were high susceptible tobacterial wilt disease, but Santana and Valor cultivars were moderately susceptible and/or resistance to thedisease. Also, Diamont and Lady balfor cultivars appeared resistance to bacterial wilt disease. Proteomicprofiles were very different for protein band density in comparison between healthy and infected potatocultivars. Genetically, seven RAPD primers gave polymorphism with the studied genotypes using RAPD-PCRtechnique. Only three primers exhibited molecular markers among these genotypes. Where, three molecularmarkers were detected in primer (C20); first was positive marker linked with susceptible (S) cultivars, meanwhile,second was detected positively with moderate and/or susceptible (M/S) cultivars to (B.W) and negatively withresistance (R) cultivars. Only one positive marker was detected in primer (A14) liked with R cultivars. Twomolecular markers were detected in Primer (C11) positively liked with R cultivars. Genetically, these cultivarswere divided to three cluster groups, Diamont and Lady blfor in the first cluster as R cultivars and Santana andValor in the second cluster as M/S cultivars, Nicola and Lady rosetta were in the third cluster as S cultivars.Using one SRAP1 primer for sequence related amplification polymorphism in studied potato cultivars by SRAP-PCR technique gave polymorphism and showed positive and negative markers explained the interactionbetween different exons for susceptibility or resistance of potato cultivars with B.W.

(PDF) Molecular Markers Associated with Some.... Available from: https://www.researchgate.net/publication/296486875_Molecular_Markers_Associated_with_Some_Resistance_Potato_Cultivars_to_Bacterial_Wilt_Disease [accessed Jul 18 2018].