Purification and Structure Elucidation of Potential Cytotoxic Agents from Streptomyces parvus KJ623765
Aboshanab, Khaled; Ahmed Said Abu Zaid; Mahmoud Abdel Magead Yassin; Nadia Abdel-Haleim Hassouna;
Abstract
Fermentation of Streptomyces (S.) parvus (accession code KJ623765), a locally isolated Streptomyces strains
from Egyptian soil sample, was carried out in a 14 L laboratory fermentor. The optimum fermentation
conditions were; incubation temperature, 28°C, agitation, 200 rpm, aeration, 5 standard liters per minute
(SLPM), 2 bar airflow pressure, uncontrolled pH. After 72 h, the cell-free culture supernatant (CFCS) was
collected and extracted using ethyl acetate (1:1, v/v) at pH 7.0. The crude extract showed potential cytotoxic
activities against Caco2 and HeLa cancer cell lines with CD50 of 3.4 and 6.8 μg/ml, respectively. Bioassay
guided fractionation had led to the purification of compounds FF1 (12 mg), FGH4 (35 mg) and FGH5 (42 mg)
per liter of the CFCS. Based on mass, 1D and 2D NMR spectroscopic analysis, they were identified as
thymidine, diadzein and genistein, respectively. Production improvement was done using gamma radiation,
where fourteen mutants showed marked increase in cytotoxic activity that reached 9.4 fold increase with
mutants P43 and P59. Accordingly, S. parvus KJ623765 can be used as an industrial isolate for the commercial
production of important cytotoxic metabolites. It is the first report about production of these anti-proliferative
metabolites by the respective isolate.
from Egyptian soil sample, was carried out in a 14 L laboratory fermentor. The optimum fermentation
conditions were; incubation temperature, 28°C, agitation, 200 rpm, aeration, 5 standard liters per minute
(SLPM), 2 bar airflow pressure, uncontrolled pH. After 72 h, the cell-free culture supernatant (CFCS) was
collected and extracted using ethyl acetate (1:1, v/v) at pH 7.0. The crude extract showed potential cytotoxic
activities against Caco2 and HeLa cancer cell lines with CD50 of 3.4 and 6.8 μg/ml, respectively. Bioassay
guided fractionation had led to the purification of compounds FF1 (12 mg), FGH4 (35 mg) and FGH5 (42 mg)
per liter of the CFCS. Based on mass, 1D and 2D NMR spectroscopic analysis, they were identified as
thymidine, diadzein and genistein, respectively. Production improvement was done using gamma radiation,
where fourteen mutants showed marked increase in cytotoxic activity that reached 9.4 fold increase with
mutants P43 and P59. Accordingly, S. parvus KJ623765 can be used as an industrial isolate for the commercial
production of important cytotoxic metabolites. It is the first report about production of these anti-proliferative
metabolites by the respective isolate.
Other data
Title | Purification and Structure Elucidation of Potential Cytotoxic Agents from Streptomyces parvus KJ623765 | Authors | Aboshanab, Khaled ; Ahmed Said Abu Zaid ; Mahmoud Abdel Magead Yassin ; Nadia Abdel-Haleim Hassouna | Keywords | Bioassay guided fractionation; Cytotoxic activity; diadzein; Fermentation; Genistein; Streptomyces parvus; thymidine. | Issue Date | 2017 | Publisher | World Journal of Pharmaceutical Sciences | Journal | World Journal of Pharmaceutical Sciences |
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