Effects of plasmid acquisition by Pseudomonas aeruginosa clinical isolates on methicillin-resistant Staphylococcus aureus biofilm formation

Aboshanab K.M.A; Nadia A. Hassouna; Mohammad M. Aboulwafa; Sarra E. Saleh;

Abstract


This work aimed to study the influence of plasmid acquisition on the mutual interaction of certain
Pseudomonas aeruginosa clinical isolates and methicillin resistant Staphylococcus aureus (MRSA)
clinical isolates. In this study, two mixed cultures were used: i) P. aeruginosa clinical isolate P14 which
co-existed with MRSA isolate S14; and ii) P. aeruginosa clinical isolate P12 which co-existed with
MRSAisolate S12. The respective mixed cultures were selected to study the existence of the plasmids
on the inhibitory effect of the culture supernatant of P. aeruginosa on the biofilm formation of coexisting
MRSA clinical isolates. The culture supernatant of P. aeruginosa harboring no plasmids
exerted a significant reduction effect on the biofilm formation (about 57% reduction; P < 0.005) of the
co-existing MRSA isolate and such effect was also confirmed using plasmid transformation
experiments. To ensure that such effect was habitually due to the presence of plasmids and not to any
mutational or conformational changes in the gene/protein sequences of the key genetic elements
involved in interspecies interaction, the genes, araC, pqsS and ahlScoded for AraC family
transcriptional regulator (1026 bp, 341 aa), and 2-heptyl-3-hydroxy-4(1H)-quinolone synthase (1149 bp,
382 aa) and acyl-homoserine-lactone synthase (540 bp; 179 aa) of P. aeruginosa clinical isolate P14
were amplified using PCR, analyzed and submitted into the GenBank database under the accession
codes, KT693035, KT693034, KT693033, respectively. AraC was a model of AraC transcription regulator
with a conserved N-terminal arabinose-binding domain and C-terminal H-T-H motive. PqsS was a model
of putative 2-heptyl-3-hydroxy-4(1H)-quinolone synthase with a conserved domain of a NAD(P)-binding
Rossmann-like domain. AhlS showed a conserved domain with the acyl-homoserine-lactone synthase
(LasI) of the protein family COG3916. The open reading frames (ORFs) of the respective genes showed
no mutation or deviation in the predicted tertiary structures.Inconclusion, plasmid acquisition
significantly decreased the inhibitoryeffect of P. aeruginosa on the biofilm formation of the co-existing
MRSA isolates.


Other data

Title Effects of plasmid acquisition by Pseudomonas aeruginosa clinical isolates on methicillin-resistant Staphylococcus aureus biofilm formation
Authors Aboshanab K.M.A ; Nadia A. Hassouna ; Mohammad M. Aboulwafa ; Sarra E. Saleh 
Keywords Arabinose-binding transcription regulator, 2-heptyl-3-hydroxy-4(1H)-quinolone synthase, acylhomoserine- lactone synthase, Pseudomonas aeruginosa, methicillin resistant Staphylococcus aureus (MRSA), biofilm, Quorum sensing.
Issue Date 2015
Journal African Journal of Microbiology Research 
DOI 10.5897/AJMR2015.7789

Attached Files

File Description SizeFormat Existing users please Login
sara saleh AJMR.pdf1.43 MBAdobe PDF    Request a copy
Recommend this item

Similar Items from Core Recommender Database

Google ScholarTM

Check

views 23 in Shams Scholar
downloads 3 in Shams Scholar


Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.