Potential ameliorative effect of vitamin E against Diazinon induced oxidative stress and hepato-renal toxicity in rats

Abstract

Diazinon (DZN) an organophosphorus pesticide ,which has led to a variety of negative effects in non target species including human. Vitamin E is a primary antioxidant that plays an important role in protecting cells against toxicity following pesticides exposure. Therefore, the present study was undertaken to investi¬gate the possible protective effect of vitamin E against DZN- induced hepato-renal toxicity in rats. Oral DZN administration induced a significant increase in serum transaminases (AST & ALT), alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) activities . Also, urea and creatinine levels were significantly increased in DZN treated group, whereas there is no significant difference in serum sodium and potassium levels in experimental groups. Moreover, the significantly increased oxidative stress biomarkers as Malondialdehyde ( MDA )and nitric oxide(NO) levels and the decreased antioxidant defenses like reduced glutathione (GSH) level and antioxidant enzymes as catalase (CAT) and superoxide dismutase (SOD) activities were noted in hepatic and renal tissues of DZN-treated group. The results showed that co-administration of vitamin E with DZN attenuated oxidative damage in DZN treated group and normalize renal and hepatic markers .It can be concluded that vitamin E supplementation has an ameliorative effect against DZN induced oxidative stress and hepato-renal toxicity .