The effect of different doses of exogenous enzymes preparation on in vitro gas production and ruminal fermentation activities of some fibrous feeds in cows

Hany M. Gado ; M. M. Y. Elghandour ; C.G. Peñuelas-Rivas ; M. Ronquillo ; A. Z. M. Salem ; N. E. Odongo 


This study was conducted to investigate effects of different doses of exogenous enzyme preparation (ENZ- 0, 0.06, 0.12 and 0.24 g/g DM) on in vitro gas production and some ruminal fermentation patterns such as truly degraded substrate (TDS), short chain fatty acids (SCFA), and microbial protein production (MP) of 4 fibrous feeds (FF - Sugarcane leaves, taiwan grass, sorghum straw and llanero grass) in cows. Rumen liquor was collected before the morning feeding from 2 cows (450 ± 6 kg BW) fitted with permanent rumen cannula. In vitro gas production (GP) was recorded at 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of incubation. After 72 h, the incubation was stopped and the inoculants pH was determined and filtered to determine TDS. Ruminal fermentation parameters such as 24 h partitioning factor (PF24), gas yield (GY24), in vitro organic matter digestibility (IVOMD), metabolizable energy (ME), SCFA, and MP were also estimated. Data were analyzed as 4 (4 doses of ENZ) x 4 (4 FF) factorial experimental design. Interaction between FF × ENZ (P < 0.05) only occurred for gas production at 12 h (GP12) and 24 h (GP24) of incubation, but there were no interactions after 24 h of incubation. Relative to control, addition of ENZ increased (P < 0.05) accumulated gas volume of all FF during the 72 h of incubation compared with control (0 g ENZ/g DM). Among the FF species, gas production was higher (P < 0.001) in sorghum straw and lower (P < 0.001) in sugarcane leaves. There was no significant effect of ENZ on gas production parameters (i.e., b; asymptotic gas production, c; rate of gas production and L; discrete lag time before gas production) of all FF. Addition of ENZ doses increased (P < 0.05) gradually gas production and this increase was higher (P < 0.05) for 0.24 g ENZ/g DM. addition of ENZ was increased (P < 0.05) some fermentation parameters (i.e., IVOMD, ME, GY24 and SCFA) were higher (P < 0.05) of all FF, while the highest (P < 0.05) increased were in sorghum straw with a lower values in sugarcane leaves. Results indicated that exogenous enzymes preparation probably improve the ruminal fermentation activities of fibrous feeds in cows. The authors wish to acknowledge the financial support from IAEA/FAO project MEX 16307.

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Keywords Exogenous enzymes;fibrous feeds;Gas production
Issue Date 2012
Publisher Annual meeting of ASAS & ADSA 2012
Journal Annual meeting of ASAS & ADSA 2012 

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