Electron microscoplcal study of the possibility of recovery of the kidney of albino mice following the injecton by the of NSAID Diclofenac sodium (rheumarene).

Abstract

the NSAIDs are heterogenous groap of chemical compounds , being useful for the prophylaxis of many cases of rheumatoid arthritis , osteoarthritis and other inflammatory disorders .Nonethless , many reports referred to their deleterious effects on some body organs . The present investigation aims to illustrate the noxious impacts following the administration the NSAID Rheumarene on the ultrastructure of the kidney of albino mice as well as the possibility of recovery or generation after drug abstinence Fifty adult male albino mice were divided into five groups . Group I served as a control group .Group II was injected intramuscularly with the therapeutic dose therapeuti(1.5mg/kg.body weight )of the NSAID rheumarene for two weeks .Group III was treated with the therapeutic dose of the NSAID RHEUMARENE for three weeks .Group IV was injected with double the therapeutic dose of the NSAID Rheumarene for three weeks .Group V was given the therapeutic dose for three weeks for further 6 weeks in order to see the possibility of recovery after drug withdrawal .After each treatment period , the kidney specimens were processed , examined by TEM and showed the following results .The renal corpuscles illustrated devastation of the parietal epithelial cells of Bowman,s capsules , dilation of the glomerular capillaries and focal fusion of the podocytes secondary foot processes .The proximal and distal convoluted turbular cells showed erosion of the microvilli of their apical borders and aggregation of hyaline and cellular casts in the tubular lumen , their mitochondria showed obvious swelling in group II .The results were more extensive in treated groups III & IV . Nonetheless , in recovery group (V) , the renal corpuscles partially restored their normal constriction in spite of the presence of deteriorated glomerular capillaries . The PCT & DCT cells manifested rather normal microvilli of their apical borders and few tubular casts were still located in their lumina . Their cytoplasm showed deteriorated mitochondria as well as some lysosomes . In conclusion : Such alterations of the ultrastructure of the kidney are not easy to recover rapidly after drug withdrawal and it may take very long period to attain complete regeneration.