EFFECT OF RANITIDINE (HISTAMINE H2-ANTAGONIST RECEPnTOR) DRUG ON SOME JISTOLOGICAL AND HISTOCHEMICAL ASPECTS OF THE MOUSE LIVER

Abstract

ABSTRACT The present investigation was designed to access the effect of one of the Histamine H2-antagonist receptor drugs known as “Ranitidine” on the liver of albino mice from the hstological and histochemical point of views. Four groups of mice were used in the present study. Group (I) served as a control group, while groups (II) and (III) were subjected to a daily oral dose of 4 mg or 8 mg Ranitidine/kg body weight respectively for 4 weeks. Mice of group (IV) were daily administrated with 4 mg Ranitidine/kg. body weight for 8 weeks. The histological findings illustrated in the hepatic tissues of group (II) indicated certain degrees of degeneration of the hepatic cells including cloudy swelling, nuclear pyknosis and karyolysis. The hepatic sinusoids were dilated and the blood vessels were congested with blood. Moreover, activated Kuptter cells were noticed and extravagated blood cells were extent to the hepatic tissue. Marked deleterious consequences were recorded in the mouse liver of group (III). The hepatocytes manifested obvious festures of hydrobic, and fatty degenerations and their nuclei showed variable degrees of pyknosis and karyolysis. The blood vasculatures were seriously devastated and contained stagnant extravasated hemolyzed blood and prominent interlobular edema. The highest degree of severity induced by the drug was observed in the hepatic tissues of mice from group (IV). The hepatocytes illustrated marked neerrobiosis, with obvious cytolysis and severe karyolysis. The blood vasculature were damaged allowing extravasation of their erythrocytes. The Kupffer cells migrated towards the lumina of the deteriorated sinusoids, and focal areas of inflammatory infiltration with leucocytes were recorded. The histochemical data displayed dose and time dependent diminution of glycogen and proteinic inclusions of the hepatic cells in the treated animals as compared with the control ones. More glycogen depletion were noticed in the lobular hepatic cells than the peripheral and cenrolobular cells. A gradual increase in the lipid inclusions was noticed in the hepatic cells of the treated groups.