Biochemical Studies on Culex pipiens (L.) (Diptera: Culicidae) Exposed to Allium satvium, Citrus limon and Bacillus thuringiensis israelensis with reference to Assessment of the Biosafety on Albino Mice

Abstract

The present work studied the electrophoretic banding pattern of untreated and treatedCulex pipiens larvae with plant extracts (Allium satvium, Citrus limon) and Bacillus thuringiensis israelensis and assessment of biosafety of these extracts and bacteria to non-target-organism were also undertaken. A total of 13 protein bands were detected in the untreated larvae. However, after exposure to 10 ul garlic oil extracts, at 12 hrs, the larval SDS dissociated protein was separated into 18 protein bands with 2 shared common protein bands. At 24 hrs post exposure, there were 17 protein bands were detected showing common 3 protein bands shared with the control. Moreover,, at 48 hrs, the numbers of bands recorded were 15 protein bands with 3 shared common protein bands with control. Among treatment with lemon oil, at 12, 24, and 48 hrs. post exposure, the larval SDS dissociated proteins were separated into 9,7,8 protein bands, respectively. These bands included three shared common protein bands with control. While after exposure to 5 ul of Bacillus thuringiensis israelensis. The total number of protein bands, at 30, 90 and 150 minutes was 8,9 and 9 bands, respectively. There were 4, 5 and 7 shared protein bands with control respectively. Protein pattern of treated fourth instar Culex pipiens larvae differ not only between various dosages but also between various time intervals at the same dosage. Intraperitonealy injection of BALB/c strain albino mice with two types of plant oil extract andBacillus thuringiensis israelensis caused no marked effect on serum alanine transaminase and asparate transaminase, cholesterol, triglycerides, total protein, bilirubin and globulin. It was concluded that the use of plant oil extracts ( Allium satvium, Citrus limon) and Bacillus thuringiensis israelensis have great effect on the total protein of Culex pipiens larvae with no effect on serum of mice, suggesting that they may be a new promising controlling agents.