Using of paramagnetic nanoparticales with immunomagnetic bead ELISA in diagnosis of hydatidosis (Echinococcus Granulosus)
ahmed, walaa; Reem O. Ahmed; Ibrahim Rabee; Eglal A. Koura;
Abstract
Cystic echinococcosus (CE) is a serious public health problem in sheep-raising regions. Detection of E.
granulosus antigens is a better immunodiagnostic tool than determination of the antibody level. The circulating
protoscolex antigen (CPA) used was obtained from lung and liver cysts of sheep and camel and injected in
rabbits to raise specific polyclonal antibodies (pAb) against E. granulosus. A novel immunomagnetic bead
ELISA based on immunoglobulin G (IgG) for detection of CPA in sera of rabbit infected with E. granulosus
was developed. Detection of CPA in serum of human patients by sandwich ELISA gave a sensitivity of 90.48%
and a specificity of 91.3% compared to other parasitic infections group and 100% compared to healthy control
group. On the other hand, detection of CPA in serum of human patients by sandwich ELISA with paramagnetic
nanoparticles gave a sensitivity of 95.2% and a specificity of 95.5% compared to other parasitic infections group
and 100% compared to healthy control group. In conclusion, sandwich ELISA with paramagnetic nanoparticles
techniques appears to be sufficiently sensitive assays for the detection of human echinococcosis antigen and
valuable to judge the efficacy of chemotherapy than sandwich ELISA.
granulosus antigens is a better immunodiagnostic tool than determination of the antibody level. The circulating
protoscolex antigen (CPA) used was obtained from lung and liver cysts of sheep and camel and injected in
rabbits to raise specific polyclonal antibodies (pAb) against E. granulosus. A novel immunomagnetic bead
ELISA based on immunoglobulin G (IgG) for detection of CPA in sera of rabbit infected with E. granulosus
was developed. Detection of CPA in serum of human patients by sandwich ELISA gave a sensitivity of 90.48%
and a specificity of 91.3% compared to other parasitic infections group and 100% compared to healthy control
group. On the other hand, detection of CPA in serum of human patients by sandwich ELISA with paramagnetic
nanoparticles gave a sensitivity of 95.2% and a specificity of 95.5% compared to other parasitic infections group
and 100% compared to healthy control group. In conclusion, sandwich ELISA with paramagnetic nanoparticles
techniques appears to be sufficiently sensitive assays for the detection of human echinococcosis antigen and
valuable to judge the efficacy of chemotherapy than sandwich ELISA.
Other data
Title | Using of paramagnetic nanoparticales with immunomagnetic bead ELISA in diagnosis of hydatidosis (Echinococcus Granulosus) | Authors | ahmed, walaa ; Reem O. Ahmed ; Ibrahim Rabee ; Eglal A. Koura | Keywords | Echinococcosis – Immunomagnetic bead ELISA technique – Paramagnetic nanoparticles -Circulating protoscolex antigen (CPA) | Issue Date | 28-Nov-2015 | Publisher | Atom and Cell Publishers | Journal | World Journal of Pharmaceutical Sciences |
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