Mechanisms of Resistance to Erythromycin among Viridans Group Streptococci (VGS) Isolated from Blood Cultures
Magda S. Gabr; Samar S. Rashad; Rania A. Amar; Moataz B. El Noamany;
Abstract
The aim
of this work was to evaluate erythromycin resistance mechanisms
of
viridans group
streptococci (VGS) isolates obtained
from
blood
cultures. Fifty
VGS isolates were obtained from
patients suffering from
infective endocarditis as
suggested by their primary physicians. The macrolide resistance mechanisms of
erythromycin resistant isolates were studied by double disc test and real time PCR.
Fifteen (30%) VGS isolates resistant to erythromycin were distributed genetically
as fo
llows:
erm
B (53.3%),
erm
B+
mef
A/E (13.3%) (Those isolates containing
erm
B
whether alone or combined with
mef
A/E
account for MLS
B
phenotype),
mef
A/E
(26.6%)
(account for M
phenotype) and only phenotypically (6.8%)
in rank of
order.
From
our study, we concluded that constitutive MLS
B
phenotype associated
with
erm
B
was the predominant mechanism
of macrolide resistance among our
erythromycin resistant VGS isolates and M phenotype was associated with
mef
A/E.
of this work was to evaluate erythromycin resistance mechanisms
of
viridans group
streptococci (VGS) isolates obtained
from
blood
cultures. Fifty
VGS isolates were obtained from
patients suffering from
infective endocarditis as
suggested by their primary physicians. The macrolide resistance mechanisms of
erythromycin resistant isolates were studied by double disc test and real time PCR.
Fifteen (30%) VGS isolates resistant to erythromycin were distributed genetically
as fo
llows:
erm
B (53.3%),
erm
B+
mef
A/E (13.3%) (Those isolates containing
erm
B
whether alone or combined with
mef
A/E
account for MLS
B
phenotype),
mef
A/E
(26.6%)
(account for M
phenotype) and only phenotypically (6.8%)
in rank of
order.
From
our study, we concluded that constitutive MLS
B
phenotype associated
with
erm
B
was the predominant mechanism
of macrolide resistance among our
erythromycin resistant VGS isolates and M phenotype was associated with
mef
A/E.
Other data
| Title | Mechanisms of Resistance to Erythromycin among Viridans Group Streptococci (VGS) Isolated from Blood Cultures | Authors | Magda S. Gabr ; Samar S. Rashad ; Rania A. Amar ; Moataz B. El Noamany | Keywords | VGS, Blood cultures, Erythromycin resista nce, MLS B phenotype, M phenotype, erm B, mef A/E | Issue Date | Nov-2015 | Publisher | international journal of current microbiology and applied sciences | Journal | International Journal of Current Microbiology and Applied Science |
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