Vitamin D status and its modulatory effect on interferon gamma and interleukin-10 production by peripheral blood mononuclear cells in culture
soliman, Dina; Dina Ragab; Dalia Samaha; Aisha Yassin;
Abstract
We aimed to investigate the influence of vitamin D on the production of the pro-inflammatory
cytokine, interferon gamma (IFN-c), and the anti-inflammatory cytokine, interleukin-10 (IL-10), in
peripheral blood mononuclear cell (PBMC) cultures.
Methods: Thirty healthy subjects were investigated. Serum levels of calcium, 25(OH) D, and parathyroid
hormone (PTH) were assessed. PBMCs were activated in-vitro by phytohemagglutinin (PHA) in the presence
and absence of vitamin D3 and then levels of IFN-c and IL-10 were determined in culture supernatant
using enzyme immunoassay.
Results: Serum calcium levels were significantly lower in the vitamin D deficient group while serum PTH
levels were significantly higher in the vitamin D deficient group. PTH levels were inversely correlated to
both calcium and 25(OH) D levels. In culture, vitamin D inhibited IFN-c production and increased IL-10
production by PBMCs. Serum vitamin D status had no influence on the amount of cytokine produced in
culture.
Conclusions: This study demonstrates that vitamin D modulates IFN-c and IL-10 production and provides
a rationale for evaluating vitamin D as an immunomodulatory agent.
cytokine, interferon gamma (IFN-c), and the anti-inflammatory cytokine, interleukin-10 (IL-10), in
peripheral blood mononuclear cell (PBMC) cultures.
Methods: Thirty healthy subjects were investigated. Serum levels of calcium, 25(OH) D, and parathyroid
hormone (PTH) were assessed. PBMCs were activated in-vitro by phytohemagglutinin (PHA) in the presence
and absence of vitamin D3 and then levels of IFN-c and IL-10 were determined in culture supernatant
using enzyme immunoassay.
Results: Serum calcium levels were significantly lower in the vitamin D deficient group while serum PTH
levels were significantly higher in the vitamin D deficient group. PTH levels were inversely correlated to
both calcium and 25(OH) D levels. In culture, vitamin D inhibited IFN-c production and increased IL-10
production by PBMCs. Serum vitamin D status had no influence on the amount of cytokine produced in
culture.
Conclusions: This study demonstrates that vitamin D modulates IFN-c and IL-10 production and provides
a rationale for evaluating vitamin D as an immunomodulatory agent.
Other data
Title | Vitamin D status and its modulatory effect on interferon gamma and interleukin-10 production by peripheral blood mononuclear cells in culture | Authors | soliman, Dina ; Dina Ragab ; Dalia Samaha ; Aisha Yassin | Keywords | Vitamin D IFN-c IL-10 | Issue Date | 2016 | Publisher | journal homepage: www.journals.elsevier.com/cytokine | Journal | Cytokine 85 (2016) 5–10 | DOI | http://dx.doi.org/10.1016/j.cyto.2016.05.024 1043-4666/ 2016 Elsevier Ltd. |
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