TH1 cytokine response to HCV peptides in Egyptian health care workers: a pilot study
soliman, Dina; Mona Rafik; Alaa El-Dien MS Hosny; Amal A Abbas; Khaled O Abdallah; Khaled M Nasr El-Din Rakha2; Rania A Abo Shady; Shahira F Alfedawy;
Abstract
Our objective was to elucidate the effects of different HCV peptides on TH1 cytokine synthesis (interleukin 2(IL2),
gamma interferon (INFγ) and tumor necrosis factor α (TNF α)), in a proliferative response in a high risk population of
HCV seronegative aviremic Egyptian healthcare workers (HCW). We studied the TH1 cytokine response to different
HCV peptides among 47 HCW with and without evidence of HCV infection. Participants were classified according to
the proliferation index (PI) in a CFSE proliferation assay as an indicator of previous exposure to HCV. Cytokines were
analyzed using Luminex xMAP technology. Results showed that positive PI HCW produced a higher IL2 in response
to all HCV peptides except NS4, a higher IFNγ response to NS3 and NS4 and no difference in TNFα response when
compared to the negative PI HCWs. When compared to chronic HCV HCW, positive PI HCW showed no difference
in the IL2 response, a higher IFNγ response to NS4 and NS5 HCV peptides and a higher TNFα response to all
peptides. In conclusion the magnitude and type of cytokines produced in HCV infection is critical in determining
the outcome of infection. NS4 & NS5 HCV peptides induce a protective TH1 response in positive PI HCW
gamma interferon (INFγ) and tumor necrosis factor α (TNF α)), in a proliferative response in a high risk population of
HCV seronegative aviremic Egyptian healthcare workers (HCW). We studied the TH1 cytokine response to different
HCV peptides among 47 HCW with and without evidence of HCV infection. Participants were classified according to
the proliferation index (PI) in a CFSE proliferation assay as an indicator of previous exposure to HCV. Cytokines were
analyzed using Luminex xMAP technology. Results showed that positive PI HCW produced a higher IL2 in response
to all HCV peptides except NS4, a higher IFNγ response to NS3 and NS4 and no difference in TNFα response when
compared to the negative PI HCWs. When compared to chronic HCV HCW, positive PI HCW showed no difference
in the IL2 response, a higher IFNγ response to NS4 and NS5 HCV peptides and a higher TNFα response to all
peptides. In conclusion the magnitude and type of cytokines produced in HCV infection is critical in determining
the outcome of infection. NS4 & NS5 HCV peptides induce a protective TH1 response in positive PI HCW
Other data
Title | TH1 cytokine response to HCV peptides in Egyptian health care workers: a pilot study | Authors | soliman, Dina ; Mona Rafik ; Alaa El-Dien MS Hosny ; Amal A Abbas ; Khaled O Abdallah ; Khaled M Nasr El-Din Rakha2 ; Rania A Abo Shady ; Shahira F Alfedawy | Keywords | CFSE, Proliferation assay | Issue Date | 2013 | Publisher | licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | Source | Rafik et al.: TH1 cytokine response to HCV peptides in Egyptian health care workers: a pilot study. Virology Journal 2013 10:144. Submit | Journal | Virology Journal 2013, 10:144 http://www.virologyj.com/content/10/1/144 | DOI | 10.1186/1743-422X-10-144 |
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