Gene Over Expression of Glycerol-3-phosphate Dehydrogenase in Some Marine Algae of Egypt

emam, Manal ; H.A. Mansour ; A.M. Shaaban ; N. H. Mostafa ; M.M. Emam 


Abstract


TUDIES focusing a survey or a comparison between freshwater …….and marine algae are relatively scarce. Some biochemical comparative study between three freshwater algae (Spirogyra reinhardii, Cladophora glomerata (green) and Chara vulgaris and six marine algae among them brown: Sargassum dentifolium, Padina boryana, Dictyota dichotoma; red: Gelidium latifolium, Gracilaria dura and green: Enteromorpha intestinalis) has been performed to study how marine algae can cope with salinity of seawater. Results obtained showed that fluorescence peaks were relatively uniform for the studied species within the same class but were different for different classes or divisions. The studied protein profile revealed the presence of three common protein bands (125, 15 and 8 kDa) only in the members of euhalopic algal group and finally the presence of one common protein band (240 kDa) only in all members of oligohalopic algae. The amount of proline showed irregular differences among the investigated marine and fresh algae. Mannitol was detected only in the members of brown algae. Moreover, the minerals (sodium and potassium) and glycerol contents of the marine algae have noticeable greater values than those of fresh algae. Glycerol-3-phosphate dehydrogenase (G3PDH) had a significant impact in the biosynthesis of glycerol. In the present study we explore the differential expression of (G3PDH) in marine algal group as compared to that of freshwater one. Results showed that, the expression level of (G3PDH) mRNA was significantly over expressed in marine algal group.


Other data

Keywords Marine algae, Freshwater algae, Flourescence emission, spectra, SDS-PAGE or protein profile, Mannitol, Proline,Minerals content, Glycerol-3-phosphate dehydrogenase.
Issue Date 2017
Journal Egypt. J. Bot 
URI http://research.asu.edu.eg/handle/123456789/169939


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