Different Stages of Hyperthyroidism: Alterations in Proliferation, Apoptosis, and Histology of Female Rat Ovary

Taha, AlShaimaa ; Nahla S. Hassan ; Manal M. Elbandrawy ; Ehab M. Tousson 


The effects of different stages of hyperthyroidism on female estrous cyclicity, mediators of inflammation, proliferation, and apoptosis in rats’ ovaries were evaluated in this study. Mild, moderate, and severe hyperthyroidism were induced in adult female rats by daily oral administration of L-thyroxin for 10, 20, and 30 days, respectively. Serum levels of thyroid stimulating hormone (TSH), free triiodotyrosine (FT3), progesterone, and estradiol II were assayed. The ovarian levels of interleukin-6 (IL-6) and insulin-like growth factor-1 (IGF-1) were also estimated. The localization and expression of both proliferating cell nuclear antigen (PCNA) and BCl-2 were evaluated in ovaries by immunohistochemistry. Ovarian and uterine histological alterations were examined microscopically. Mild hyperthyroidism did not change serum level of estradiol II but decreased significantly serum progesterone level, while moderate and severe hyperthyroidism decreased significantly the serum level of the two hormones. Mild hyperthyroidism did not alter the ovarian and uterine histological structure, ovarian IL-6 concentration, and expressions of PCNA and BCl-2, but it produced a slight reduction in the ovarian IGF-1 concentration. Increasing the severity of hyperthyroidism produced ovarian inflammation and apoptosis, which is evident from the measured parameters and the histological changes. In conclusion, moderate and severe hyperthyroidism altered estrous cyclicity, inflammatory, proliferative, and apoptotic markers in rats’ ovary.

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Keywords Hyperthyroidism - Apoptosis - Ovary - Proliferation
Issue Date Jun-2018
Journal Research Journal of Pharmaceutical, Biological and Chemical Science 
URI http://research.asu.edu.eg/handle/123456789/170064
ISSN 0975-8585

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