Ziziphus spina-christi leaves methanolic extract alleviates diethylnitrosamine-induced hepatocellular carcinoma in rats

Taha, AlShaimaa ; Manar Salah El-Din ; Ahmed Abdel-Aziz Sayed ; Ahmed Mohamed Salem 


Abstract


The present study aimed to evaluate the antitumor activity of Ziziphus spina-christi leaves extract (ZSCL) against diethylnitrosamine (DENA)-induced hepatocarcinogenesis in rats. The phytochemical constituents, in vitro antioxidant and cytotoxic activities of ZSCL extract were investigated. Male Wistar rats were divided into 6 groups: normal control, ZSCL1-administered rats (100 mg/ Kg body weight “b.w.”), ZSCL2-administered rats (300 mg/ Kg b.w.), DENA-induced hepatocarcinogenesis, hepatocarcinogenesis-bearing rats treated either with ZSCL1 or ZSCL2. Serum liver function & oxidative stress tests were assayed. The expression of hepatocyte growth factor, insulin-like growth factor-1 receptor, B cell lymphoma-2, and matrix metalloproteinase-9 oncogenes were quantified in liver. Histological examination of liver tissues was performed. ZSCL methanolic extract was rich in essential fatty acids, phytol, and polyphenolic flavones (luteolin and quercetin) with strong free radical & peroxide scavenging activities and cytotoxic activity. Administration of ZSCL1 and ZSCL2 to rats did not alter the studied parameters. DENA induced hepatocellular carcinoma and cholangioma by producing oxidative stress and upregulating liver oncogenes expression. Treatment of DENA-induced hepatocarcinogenesis with ZSCL2 ameliorated all the abnormalities induced by DENA except for cholangioma. In conclusion, ZSCL methanolic extract (300mg/ Kg b.w.) possessed a strong therapeutic activity against DENA-induced hepatocellular carcinoma via targeting oxidative stress and oncogenes.


Other data

Keywords Ziziphus spina-christi; Leaves; Hepatocarcinogenesis; Oxidative Stress; Oncogenes
Issue Date Nov-2018
Publisher Canadian Science Publishing
Journal Biochemistry and cell Biology 
URI http://research.asu.edu.eg/handle/123456789/170065
ISSN 0829-8211


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