Characterization of Polysaccharopeptides from Pleurotus ostreatus Mycelium. Assessment of Toxicity and Immunomodulation in vivo
Taha, AlShaimaa; Fawzia M. Refaie; Amr Y. Esmat; Ayman S. Daba;
Abstract
Characterization of hot water polysaccharopeptides from the culture medium
of Pleurotus ostreatus mycelium, as well as the assessment of its toxicity and
immunomodulatory effects on mice, were carried out. Physicochemical studies
revealed that the structure of crude polysaccharopeptides consisted mainly of β-Dglucans
with (1→3) (1→6) glucosidic linkages, and they had a high molecular weight
of 316.26 kDa. Acute toxicity studies showed that polysaccharopeptides were not lethal
after 24 h, in mice given i.p. 854 mg/kg or less. Intraperitoneal administration of mice
with polysaccharopeptides, at doubling doses (25, 50 and 100 mg/kg), thrice weekly for
five consecutive weeks, has shown that although the lowest dose induced the maximum
endogenous alpha-interferon production (213.2%), yet it reduced significantly the
body weight gain of animals (39.56%) and exhibited a low mortality effect (12.50%) at
the end of the experiment.
of Pleurotus ostreatus mycelium, as well as the assessment of its toxicity and
immunomodulatory effects on mice, were carried out. Physicochemical studies
revealed that the structure of crude polysaccharopeptides consisted mainly of β-Dglucans
with (1→3) (1→6) glucosidic linkages, and they had a high molecular weight
of 316.26 kDa. Acute toxicity studies showed that polysaccharopeptides were not lethal
after 24 h, in mice given i.p. 854 mg/kg or less. Intraperitoneal administration of mice
with polysaccharopeptides, at doubling doses (25, 50 and 100 mg/kg), thrice weekly for
five consecutive weeks, has shown that although the lowest dose induced the maximum
endogenous alpha-interferon production (213.2%), yet it reduced significantly the
body weight gain of animals (39.56%) and exhibited a low mortality effect (12.50%) at
the end of the experiment.
Other data
| Title | Characterization of Polysaccharopeptides from Pleurotus ostreatus Mycelium. Assessment of Toxicity and Immunomodulation in vivo | Authors | Taha, AlShaimaa ; Fawzia M. Refaie ; Amr Y. Esmat ; Ayman S. Daba | Keywords | Physicochemical studies, Pleurotus ostreatus, polysaccharopeptides, serum interferon-α, toxicological studies | Issue Date | 2009 | Publisher | Berkeley, CA, U.S.A. | Journal | Micologia Aplicada International | DOI | http://www.micaplint.com/index.html http://www.micaplint.com/index.html http://www.micaplint.com/index.html |
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