Aminoglycoside Resistance Patterns of Certain Gram Negative Uropathogens Recovered from Hospitalized Egyptian Patients
Aboshanab K.; Mohamed S. Ashour; Samira M. Hamed; Walid F. Elkhatib;
Abstract
Aims: To determine resistance rates and patterns of certain uropathogens, including E.
coli, Klebsiella spp. and Pseudomonas spp., isolated from hospitalized urinary tract
infections patients, to aminoglycoside antibiotics and to detect the most prevalent plasmidmediated
aminoglycoside modifying enzymes (AMEs).
Methods: Uropathogenic isolates (150) were recovered from urine specimens of
hospitalized UTI patients in Cairo, Egypt and identified by conventional methods. The
recovered uropathogens (E. coli, Klebsiella spp. and Pseudomonas spp.) were tested for
their susceptibility to gentamicin, tobramycin, amikacin, neomycin, netilmicin, and
kanamycin by disc diffusion method. Plasmid-mediated aminoglycoside resistance was
determined by transformation experiments as well as by using plasmids as templates for
PCR screening of the AMEs-coding genes aph(3')-I, aac(6')-I, aac(3)-I, aac(3)-II and
ant(2'')-I in all resistant isolates. Results: Of a total of 150 uropathogenic clinical isolates, 110 isolates were of the above
mentioned genera and were selected for the current study. Sixty three isolates (57.2%)
were resistant to at least one aminoglycoside antibiotic. Highest and lowest resistance rates
were observed to kanamycin (53.6%) and amikacin (7.2%), respectively. The resistance
rates to gentamicin, neomycin, tobramycin and netilmicin were 33.6%, 24.5%, 23.6% and
14.5%, respectively. AMEs-coding genes were detected on the plasmids of 93.6% of
resistant isolates with prevalence rates of 53.9% for ant(2'')-I, 38% for both aac(6')-I and
aac(3)-II and 33.3% for aph(3')-I, while aac(3)-I gene was not detected in any of the tested
resistant isolates. Double and triple combinations of AMEs-coding genes were detected in
ich49.2% of resistant isolates.
Conclusion: A high prevalence of plasmid-mediated resistance to aminoglycoside
antibiotics in Gram negative uropathogens from hospitalized patients was observed.
Uropathogens may represent potential reservoirs of panaminoglycoside resistance in
hospitals, having on their plasmids combinations of AMEs-coding genes. Good infection
control measures in Egyptian hospitals together with periodic screening of prevalence rates
of different resistance genes are required.
coli, Klebsiella spp. and Pseudomonas spp., isolated from hospitalized urinary tract
infections patients, to aminoglycoside antibiotics and to detect the most prevalent plasmidmediated
aminoglycoside modifying enzymes (AMEs).
Methods: Uropathogenic isolates (150) were recovered from urine specimens of
hospitalized UTI patients in Cairo, Egypt and identified by conventional methods. The
recovered uropathogens (E. coli, Klebsiella spp. and Pseudomonas spp.) were tested for
their susceptibility to gentamicin, tobramycin, amikacin, neomycin, netilmicin, and
kanamycin by disc diffusion method. Plasmid-mediated aminoglycoside resistance was
determined by transformation experiments as well as by using plasmids as templates for
PCR screening of the AMEs-coding genes aph(3')-I, aac(6')-I, aac(3)-I, aac(3)-II and
ant(2'')-I in all resistant isolates. Results: Of a total of 150 uropathogenic clinical isolates, 110 isolates were of the above
mentioned genera and were selected for the current study. Sixty three isolates (57.2%)
were resistant to at least one aminoglycoside antibiotic. Highest and lowest resistance rates
were observed to kanamycin (53.6%) and amikacin (7.2%), respectively. The resistance
rates to gentamicin, neomycin, tobramycin and netilmicin were 33.6%, 24.5%, 23.6% and
14.5%, respectively. AMEs-coding genes were detected on the plasmids of 93.6% of
resistant isolates with prevalence rates of 53.9% for ant(2'')-I, 38% for both aac(6')-I and
aac(3)-II and 33.3% for aph(3')-I, while aac(3)-I gene was not detected in any of the tested
resistant isolates. Double and triple combinations of AMEs-coding genes were detected in
ich49.2% of resistant isolates.
Conclusion: A high prevalence of plasmid-mediated resistance to aminoglycoside
antibiotics in Gram negative uropathogens from hospitalized patients was observed.
Uropathogens may represent potential reservoirs of panaminoglycoside resistance in
hospitals, having on their plasmids combinations of AMEs-coding genes. Good infection
control measures in Egyptian hospitals together with periodic screening of prevalence rates
of different resistance genes are required.
Other data
Title | Aminoglycoside Resistance Patterns of Certain Gram Negative Uropathogens Recovered from Hospitalized Egyptian Patients | Authors | Aboshanab K. ; Mohamed S. Ashour ; Samira M. Hamed ; Walid F. Elkhatib | Keywords | Aminoglycoside antibiotics; Interpretative reading; Uropathogens; aph(3')-I; aac(6')-I; aac(3)-II; ant(2'')-I. | Issue Date | 2013 | Journal | British Microbiology Research Journal 3(4): 3(4): 678-691. |
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