) Plasmid Mediated Extended Spectrum Beta-Lactamase producing strains of Enterobacteraceae Isolated from Diabetic Foot Infections in Egypt
Aboshanab K. M.; Wafaa N. El-tayeb; Mohammad M. Aboulwafa; Noha A. Kamel;
Abstract
Background: Extended spectrum beta-lactamases (ESBLs) are heterogeneous
group of plasmid mediated β-lactamases enzymes that confer resistant to oxyimino
cephalosporins, monobactam (aztreonam) and usually show co-resistance to other
classes of antibiotics. The present was done to identify and characterize the most
common plasmid-mediated ESBL genes associated with diabetic foot infections in
Egypt.
Methods and findings: A total of 135 Gram negative bacterial isolates were recovered
from 91 diabetic foot ulcers specimens of patients attending governmental
hospitals and private clinics in Cairo, Egypt. The antibiogram analysis revealed a
remarkable high resistance pattern towards different classes of the tested antimicrobial
agents. Based on CLSI, a total of 114 isolates out of 135 were considered
potential ESBLs producer by using disc diffusion and broth microdilution screening
assays followed by double disc synergy test (DDST). 58 out of 114 isolates were
ESBLs producers and almost 14% (8 out of 58), were plasmid-mediated as determined
by plasmid extraction and transformation experiments. The majority of the
tested plasmids (6 out of 8) carried 2 or more genes on the same plasmid. The
most common combination was blaCTX-M and blaTEM (n=3/8; 37.5%), followed by
blaSHV and blaCTX-M(n=2/8; 25%) and blaCTX-M+ blaTEM+ blaSHV (n=1/8;12.5%).
One E.coli isolate harbored a plasmid (pECDF16) coding for the three ESBLs genes.
The final nucleotide sequences of blaTEM1 and blaSHV-8 were submitted to the
GenBank database under accessions coder JX976326, and JX976327, respectively.
Conclusion: High prevalence of plasmid-mediated ESBLs was detected among
DFIs in Egypt. Therefore, new guidelines should be undertaken in Egypt to limit or
prevent the misuse and abuse of antimicrobial agents
group of plasmid mediated β-lactamases enzymes that confer resistant to oxyimino
cephalosporins, monobactam (aztreonam) and usually show co-resistance to other
classes of antibiotics. The present was done to identify and characterize the most
common plasmid-mediated ESBL genes associated with diabetic foot infections in
Egypt.
Methods and findings: A total of 135 Gram negative bacterial isolates were recovered
from 91 diabetic foot ulcers specimens of patients attending governmental
hospitals and private clinics in Cairo, Egypt. The antibiogram analysis revealed a
remarkable high resistance pattern towards different classes of the tested antimicrobial
agents. Based on CLSI, a total of 114 isolates out of 135 were considered
potential ESBLs producer by using disc diffusion and broth microdilution screening
assays followed by double disc synergy test (DDST). 58 out of 114 isolates were
ESBLs producers and almost 14% (8 out of 58), were plasmid-mediated as determined
by plasmid extraction and transformation experiments. The majority of the
tested plasmids (6 out of 8) carried 2 or more genes on the same plasmid. The
most common combination was blaCTX-M and blaTEM (n=3/8; 37.5%), followed by
blaSHV and blaCTX-M(n=2/8; 25%) and blaCTX-M+ blaTEM+ blaSHV (n=1/8;12.5%).
One E.coli isolate harbored a plasmid (pECDF16) coding for the three ESBLs genes.
The final nucleotide sequences of blaTEM1 and blaSHV-8 were submitted to the
GenBank database under accessions coder JX976326, and JX976327, respectively.
Conclusion: High prevalence of plasmid-mediated ESBLs was detected among
DFIs in Egypt. Therefore, new guidelines should be undertaken in Egypt to limit or
prevent the misuse and abuse of antimicrobial agents
Other data
Title | ) Plasmid Mediated Extended Spectrum Beta-Lactamase producing strains of Enterobacteraceae Isolated from Diabetic Foot Infections in Egypt | Authors | Aboshanab K. M. ; Wafaa N. El-tayeb ; Mohammad M. Aboulwafa ; Noha A. Kamel | Keywords | ESBLs, plasmid-mediated ESBLs, Diabetic foot infections, Enterobacteriaceae | Issue Date | 2013 | Journal | Arch. Clinical microb. 4(4):1-8 | DOI | 10.3823/269 |
Attached Files
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