RAPID, SIMPLE SUSCEPTIBILITY TESTING OF CANDIDA SPECIES
Ali Ahmed Galal Ali Mostaphy Younes;
Abstract
Candidiasis represents a high-risk event in severely ill patients. Its increasing incidence in recent years is associated with extensive and prolonged use of broad-spectrum antimicrobial agents, diabetes mellitus, chronic renal failure, corticosteroids, immunosuppressive and cytotoxic drugs.
Although C.albicans remams the most common cause ofhuman candidiasis, other Candida species such as C.tropicalis, C.parapsilosis, C.glabrata, C.lusitaniea and C.krusei are more emerging as opportunistic pathogens and more resistant than C.albicans to antifungal agents. So there is an increasing need for the development of simple, rapid and accurate identification and antifungal susceptibility methods for potential fungal pathogens recovered in the clinical microbiology laboratory.
This study aimed to establish a reliable, simple and rapid method for antifungal susceptibility testing of Candida species. So, each ofE test and candifast methods were compared to NCCLS broth microdilution method as a reference method to assess Candida isolates susceptibility to different antifungal agents. In addition, the performance of new candifast test for identification of Candida to the species level was evaluated and compared with microscan identification method. Twenty-six Candida isolates were included in this study.
In our study, the over all agreement of candifast identification test with microscan was 80.8% as 3 strains were identified as C.albicans by micro can but by candifast they were identified as C.glabrata, C.tropicalis and C.lusitaniea. Also 2 strains were identified as C.albicans by candifast but by microscan they were identified as C.tropicalis.
The susceptibility of isolated Candida species to antifungal agents
was determined by broth microdilution, candifast and E test methods.
Although C.albicans remams the most common cause ofhuman candidiasis, other Candida species such as C.tropicalis, C.parapsilosis, C.glabrata, C.lusitaniea and C.krusei are more emerging as opportunistic pathogens and more resistant than C.albicans to antifungal agents. So there is an increasing need for the development of simple, rapid and accurate identification and antifungal susceptibility methods for potential fungal pathogens recovered in the clinical microbiology laboratory.
This study aimed to establish a reliable, simple and rapid method for antifungal susceptibility testing of Candida species. So, each ofE test and candifast methods were compared to NCCLS broth microdilution method as a reference method to assess Candida isolates susceptibility to different antifungal agents. In addition, the performance of new candifast test for identification of Candida to the species level was evaluated and compared with microscan identification method. Twenty-six Candida isolates were included in this study.
In our study, the over all agreement of candifast identification test with microscan was 80.8% as 3 strains were identified as C.albicans by micro can but by candifast they were identified as C.glabrata, C.tropicalis and C.lusitaniea. Also 2 strains were identified as C.albicans by candifast but by microscan they were identified as C.tropicalis.
The susceptibility of isolated Candida species to antifungal agents
was determined by broth microdilution, candifast and E test methods.
Other data
| Title | RAPID, SIMPLE SUSCEPTIBILITY TESTING OF CANDIDA SPECIES | Other Titles | اختبارات الحساسية السهلة والسريعة لأنواع الكانديدا | Authors | Ali Ahmed Galal Ali Mostaphy Younes | Issue Date | 2002 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.