Phenotypic and Genotypic Characterization of Salmonella spp. Isolated from Camel in North Western Coastal Area of Egypt
Marwa Fawzy ALI ALI El HELW;
Abstract
This study was applied on 284 samples of swabs, fecal samples
and organs which were collected from living & slaughtered dromederies
camels in north western coastal area of Egypt. Bacteriological and
biochemical tests were applied on them, 26 samples were positive to
Salmonella . The results of VITEK classified them into 22 field isolates of
salmonella spp. and 4 of them were Salmonella enteric ssp diarizonae. The
26 isolates showed positive band of invA gene.12 of them were positive to
spvA gene of S. Entritidis and 10 of them positive to fliC gene of
S.Typhimurium. All 26 isolates were resistant to spiramycin (100%) &
sensitive to ciprofloxacin, gentamicin, and amoxicillin/clavulinic. It is obvious
not all resistant isolates carry the resistant gene of tetracycline tetA and β-
lactam blaTEM & blaSHV & vice versa.SDS explained that the mol.weight of
bands were closely related with variable heterogenous banding profile. The
analysis of 16s rRNA gene of 11 isolates showed 100% similarity between
3 isolates of the same origin ,S. Entritidis and with one samples of
S.Typhimurium while the percentage of divergence was 0.2- 0.4%.
and organs which were collected from living & slaughtered dromederies
camels in north western coastal area of Egypt. Bacteriological and
biochemical tests were applied on them, 26 samples were positive to
Salmonella . The results of VITEK classified them into 22 field isolates of
salmonella spp. and 4 of them were Salmonella enteric ssp diarizonae. The
26 isolates showed positive band of invA gene.12 of them were positive to
spvA gene of S. Entritidis and 10 of them positive to fliC gene of
S.Typhimurium. All 26 isolates were resistant to spiramycin (100%) &
sensitive to ciprofloxacin, gentamicin, and amoxicillin/clavulinic. It is obvious
not all resistant isolates carry the resistant gene of tetracycline tetA and β-
lactam blaTEM & blaSHV & vice versa.SDS explained that the mol.weight of
bands were closely related with variable heterogenous banding profile. The
analysis of 16s rRNA gene of 11 isolates showed 100% similarity between
3 isolates of the same origin ,S. Entritidis and with one samples of
S.Typhimurium while the percentage of divergence was 0.2- 0.4%.
Other data
| Title | Phenotypic and Genotypic Characterization of Salmonella spp. Isolated from Camel in North Western Coastal Area of Egypt | Other Titles | التوصيف الشكلي و الجيني للسالمونيلا المعزوله من الجمال في منطقة الساحل الشمالي الغربي بمصر | Authors | Marwa Fawzy ALI ALI El HELW | Issue Date | 2018 |
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