Comparative Bacteriological and Molecular Studies on Klebsiella Species Isolated From Chicken and Human

Abstract

Klebseilla infection is considered to be one of the important diseases affecting poultry. The disease causes high mortality rate among affected birds, which sometimes result in lowering of hatchability, fertility and egg production in layers. Incidence of klebseilla among broilers, layers , baby chicks and humans was investigated in the current study. The isolates were recovered from Alexandria Governorate through microbiological examination of 1000 various samples (liver, spleen, lung and trachea) of apparently healthy birds. Fifteen Klebseilla isolates were obtained from diseased human cases (urinary tract infection, diarrhea, respiratory tract infection, wounds and tissue affections) from El Merry Hospital at Alexandria University where bacteriological and biochemical identification were carried out for further confirmation. The results showed that the incidence of klebseilla isolation among the examined broilers, layers, baby chicks and obtained human samples were 5.3% 5.8% 9.6% and 7.5%, respectively. Bbiochemical identification revealed that out of 15 chicken Klebseilla isolates, (8) were Klebseilla pneumoniae and (7) were Klebseilla oxytoca, while the 15 human isolates were identified as (7) Klebseilla pneumoniae and (8) as Klebseilla oxytoca. The antimicrobial sensitivity testing revealed that all isolates (100%) were sensitive to amikacin, cefepime and imipenem followed by (97.3%) to gentamicin, (94.6%) to aztreonam and (86.5%) to ciprofloxacin. On the other hand, (97.3%) of the isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to Sulphamethoxazole /Trimethoprim.