(MOLECULAR GENETIC STUDIES ON THE EGYPTIAN GRAY MANGROVE (AVICENNIA MARINA

Abstract

Mangrove is an ecological term for a diverse group of woody plant species that form the dominant vegetation in saline, tidal wetlands along tropical and subtropical coasts. DNA barcoding of Egyptian gray mangroves in Nabq protectorate was conducted sequencing of ITS1, ITS2. RT-PCR was conducted for four genes implicated for salt tolerance, oxidative and osmotic stresses in Egyptian gray mangroves within Nabq protectorate in South Sinai Governorate. The results showed over-expression of the mRNA of ferritin (amFer1) gene transcript, followed by slight increase in mRNA of superoxide dismutase (amSOD1) and even minute increase of ubiquitin conjugation2 (amUBC2) compared with the actin gene. At the same time gene expression of catalase (amCAT1) was down regulated. 3'-UTR gene regulation and organizational area in the four genes above, were sequenced their homologies were compared to their counterparts in five different databases, the results confirmed that the variation in the 3'-UTR region is very limited because it is harboring important regulatory region that controls the efficiency and half-life time of mRNA and thus, it is highly conserved for effective gene expression