HEPATOPROTECTIVE EFFECT OF MORINGA OLEIFERA EXTRACT AGAINST ACETAMINOPHEN INDUCED LIVER OXIDATIVE DAMAGE IN RATS

Abstract

This study aimed to evaluate the in-vitro antioxidant activities different extracts and parts of Moringa Oleifera Lam (M.O), and in-vivo study on their protective effect against acetaminophen (APAP) – induced liver injury in rats caused by oxidative damage .The antioxidant activities of ethanolic , aqueous , and hexane extracts of M.O edible parts (flowers , leaves , stem bark , roots , and seeds) were investigated by: 1,1-diphenyl-2-picryl-hydrazyl-hydrate (DPPH) radical scavenging capacity assay. The ethanolic extracts of the different parts of M.O were found to have higher antioxidant capacity compared to the aqueous and hexane extracts. The ethanolic extract of M.O flowers has the highest total antioxidant capacity among the different parts of M.O, Followed by leaves, stem bark, roots and seeds. HPLC-MS analysis for the ethanolic leaves extract showed the presence of five phenolic compounds: chlorogenic acid, Apigenin-8-C—glucoside, Quercetin-3-O-β-D-glucoside, Quercetin-3-O-acetyl glucoside and kaempferol-3-O-glucoside; these compounds are known to have antioxidant activities.