PYRIMETHAMINE TOXICITY: MODULATING EFFECT OF FOLINIC ACID
Nesrine Abdel Rahman Mahmoud;
Abstract
The effect of many chemicals on the genetic material is very important i(ll• our health. So, the toxic efi'eds or these compounds must be
carefully evaluated.
Pyrimethamine is an antimalarial agent affecting folic acid reductase synthesis. Thus, DNA replication is affected and damage of the chromosomes ensues. So, folatcs deficiency have been found to play a role in the pyrimethamine induced toxicities.
The aim of this study was first to investigate the clastogenic effects of pyrimethamine on bone marrow aspirate, other toxic effects (hepatic and CBC impact). Second, to evaluate the protective effect of folinic acid against pyrimethamine induced toxicities.
The work was carried out on 355 adult albino rats of both sex divided into three main categories of animal groups: single dose groups, repeated dose groups and control groups.
Single dose groups
Group I (pyrimethamine alone)
This was subdivided into three subgroups :
Group I (A) Consists of 20 rats, given a single oral dose of pyrimethamine (20mg/kg). This group was further subdivided into three groups:
Subgroup I A (i) Consists of 5 rats, sacrificed 3 days post treatment.
Subgroup I A ( ii)
Subgroup I A (iii)
Consists of 5 rats, sacrificed 12 days post treatment. (The previous two groups were subjected to chromosomal analysis).
Consists of I 0 rats, sacrificed 12 days post treatment for CBC estimation and biochemical analysis.
carefully evaluated.
Pyrimethamine is an antimalarial agent affecting folic acid reductase synthesis. Thus, DNA replication is affected and damage of the chromosomes ensues. So, folatcs deficiency have been found to play a role in the pyrimethamine induced toxicities.
The aim of this study was first to investigate the clastogenic effects of pyrimethamine on bone marrow aspirate, other toxic effects (hepatic and CBC impact). Second, to evaluate the protective effect of folinic acid against pyrimethamine induced toxicities.
The work was carried out on 355 adult albino rats of both sex divided into three main categories of animal groups: single dose groups, repeated dose groups and control groups.
Single dose groups
Group I (pyrimethamine alone)
This was subdivided into three subgroups :
Group I (A) Consists of 20 rats, given a single oral dose of pyrimethamine (20mg/kg). This group was further subdivided into three groups:
Subgroup I A (i) Consists of 5 rats, sacrificed 3 days post treatment.
Subgroup I A ( ii)
Subgroup I A (iii)
Consists of 5 rats, sacrificed 12 days post treatment. (The previous two groups were subjected to chromosomal analysis).
Consists of I 0 rats, sacrificed 12 days post treatment for CBC estimation and biochemical analysis.
Other data
| Title | PYRIMETHAMINE TOXICITY: MODULATING EFFECT OF FOLINIC ACID | Other Titles | سمية عقار البيريميثامين:ا لتأثير التحولى لحمض الفولينك | Authors | Nesrine Abdel Rahman Mahmoud | Issue Date | 2001 |
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