Cloning and expression of certain gene(s) putatively involved in acyl homoserine lactone quorum quenching activity as a new approach for infection control
Masarra Mohammad Abo-el-soud Sakr;
Abstract
The increasing occurrence of resistance among pathogenic bacteria like Pseudomonas aeruginosa isolates necessitates finding alternatives to antibiotics for controlling the infection of such bacteria. The present study aimed at applying quorum quenching as a new approach for infection control. In this study, lactonase gene ahl-1 from Bacillus weihenstephanensis isolate-P65 was successfully cloned and expressed in E. coli BL21 for utilizing its quorum quenching activity against multidrug resistant P. aeruginosa clinical isolates. The biological activity of the overexpressed lactonase enzyme (Ahl-1), tested using a synthetic signal and Chromobacterium violaceum CV026 as a biosensor, displayed good catalytic activity
Other data
| Title | Cloning and expression of certain gene(s) putatively involved in acyl homoserine lactone quorum quenching activity as a new approach for infection control | Other Titles | الاستنساخ و الانتاج البروتيني لبعض الجينات المفترض مسؤوليتها عن تثبيط اشارات الاستشعار العددي لمركب الاسيل هوموسيرين لاكتون كوسيلة جديدة لمكافحة العدوى | Authors | Masarra Mohammad Abo-el-soud Sakr | Issue Date | 2018 |
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