The Possible Role of Hypoxia Inducible Factor in understanding the Protective Mechanism of Remote Ischemia Pre-conditioning against Ischemia Reperfusion Injury in Isolated Hearts of Rats

Abstract

The present study was planned to elucidate the effect of the effectiveness of remote ischemia reperfusion (RIPC) in reducing myocardial Ischemia/reperfusion Injury in rats and to elucidate the possible role of hypoxia inducible factor in this protection. The study was performed on 50 albino adult female rats, allocated into 5 groups: Group I: Control rats that were subjected to ischemic /reperfusion injury only. Group II:Early RIPC rats that were subjected to RIPC 2 hours prior to ischemic /reperfusion injury. Group III:Acriflavine-treated early RIPC rats that were injectedwith acriflavine, the HIF-1alpha inhibitor (IP, 2mg/kg/day) for 9 days prior to RIPC and 2 hours prior ischemic /reperfusion injury.