BIOCHMICAL STUDIES ON JOJOBA PLANT USING TISSUE CULTURE TECHNIQUES

Abstract

This work was carried out in the tissue culture laboratory of Zohriya Garden, Department of Ornamental Plant Research, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt during the period from 2013 to 2016. Simmondsin production and its effect on hepatic cancer and breast carcinoma cells was investigated through jojoba tissue culture. Lateral buds were excised from jojoba female plants grown in Zohriya Garden. As clorox was found to be ineffective in a preliminary trial, an experiment was carried out to investigate the effect of mercuric chloride HgCl2 (MC). Three MC concentrations, 0.1, 0.2 and 0.3 g/l were applied. Buds were subjected to each concentration for three different exposure times, i.e. 5, 7 and 9 min. Survived explants that responded positively were used in another trial where they were inoculated on MS medium at different strengths, I.e. quarter, half, 3 quarters and full strength treatments.