MODELLING OF PROTEIN SEPARATION FROM GELATIN WASTEWATER USING AMMONIUM SULFATE

Abstract

Proteins are very important and have endless uses worldwide. The protein demand worldwide is increasing continuously. However, each day billions of gallons of wastewater are released domestically and industrially; the nitrogenous wastes are the worst; high percentage of nitrogenous waste in this water is proteinous wastes which contribute indeed to overall oxygen demand. Therefore, proteins, peptides and amino acids precipitation and separation from wastewater is a worth taken challenge. The objective of our thesis is to precipitate proteins present in gelatin wastewater samples; moreover, the gelatin wastewater sample underwent citric acid hydrolysis at different durations followed by solvent precipitation using ammonium sulfate (salting out). The effect of different operating conditions was considered, analyzed and optimized. The considered time from 10 to 17 hrs, temperature from 23oC to 43oC, pH from 4 to 10 and ammonium sulfate concentration from 20% to 80%.